Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
68596-1-PBS targets BCKDHB as part of a matched antibody pair:
MP50570-1: 68596-2-PBS capture and 68596-1-PBS detection (validated in Cytometric bead array)
MP50570-3: 68596-4-PBS capture and 68596-1-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag33802 Product name: Recombinant human BCKDHB protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-392 aa of BC040139 Sequence: 相同性解析による交差性が予測される生物種 |
| Full Name | branched chain keto acid dehydrogenase E1, beta polypeptide |
| Calculated molecular weight | 392 aa, 43 kDa |
| Observed molecular weight | 35-37 kDa |
| GenBank accession number | BC040139 |
| Gene Symbol | BCKDHB |
| Gene ID (NCBI) | 594 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P21953 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
Branched-chain alpha-keto acid dehydrogenase E1 component beta chain (BCKDHB; BCKDE1B; BCKDH E1-beta) is a subunit of the BCKDH complex, which is a mitochondrial enzyme in the degradation pathway for branched-chain amino acids (BCAA). Together with BCKDHA, BCKDHB forms the E1 subunit of this complex, whereas DBT and DLD are the E2 and E3 subunits, respectively. A deficiency of the BCKDH complex in humans causes maple syrup urine disease (MSUD), a severe neurometabolic disorder diagnosed by the detection alloisoleucine in plasma (MIM 248600). (PMID: 30709776)