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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BCHE Polyclonal antibody (23854-1-AP)

    WB analysis of HeLa using 23854-1-AP (same clone as 23854-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 23854-1-AP (BCHE Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23854-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using BCHE Polyclonal antibody (23854-1-AP)

    WB analysis of HEK-293 using 23854-1-AP (same clone as 23854-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23854-1-AP (BCHE Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23854-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using BCHE Polyclonal antibody (23854-1-AP)

    IHC staining of human liver using 23854-1-AP (same clone as 23854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23854-1-AP (BCHE Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 23854-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using BCHE Polyclonal antibody (23854-1-AP)

    IHC staining of human liver using 23854-1-AP (same clone as 23854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23854-1-AP (BCHE Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 23854-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BCHE Polyclonal antibody (23854-1-AP)

    IF Staining of HeLa using 23854-1-AP (same clone as 23854-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BCHE antibody (23854-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23854-1-PBS in a different storage buffer formulation.

BCHE Polyclonal antibody, PBS Only

BCHE Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 23854-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23854-1-PBS targets BCHE in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20888

Product name: Recombinant human BCHE protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 539-602 aa of BC008396

Sequence: MTKLRAQQCRFWTSFFPKVLEMTGNIDEAEWEWKAGFHRWNNYMMDWKNQFNDYTSKKESCVGL

相同性解析による交差性が予測される生物種
Full Name butyrylcholinesterase
Calculated molecular weight 602 aa, 68 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberBC008396
Gene Symbol BCHE
Gene ID (NCBI) 590
RRIDAB_2879341
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDP06276
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BCHE(butyrylcholinesterase) is an enzyme expressed in most human tissues. It may have a greater role in cholinergic transmission than previously surmised, making BChE inhibition an important therapeutic goal in Alzheimer's disease(PMID:11848688). BCHE is also named as CHE1 and belongs to the type-B carboxylesterase/lipase family. This is a glycoprotein with the molecular weight from 68 kDa to 90 kDa.

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