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Validation Data Gallery

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  • Western Blot (WB) analysis of Ramos cells using BCAT1 Monoclonal antibody (67084-1-Ig)

    WB analysis of Ramos using 67084-1-Ig (same clone as 67084-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 67084-1-Ig (BCAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67084-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using BCAT1 Monoclonal antibody (67084-1-Ig)

    WB analysis of pig brain using 67084-1-Ig (same clone as 67084-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67084-1-Ig (BCAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67084-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using BCAT1 Monoclonal antibody (67084-1-Ig)

    WB analysis of Daudi using 67084-1-Ig (same clone as 67084-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 67084-1-Ig (BCAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67084-1-PBS in a different storage buffer formulation.

BCAT1 Monoclonal antibody, PBS Only

BCAT1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67084-1-PBS
Clone No.1G3A10

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, Indirect ELISA

Synonyms

BCAT1, DKFZp686E12175, ECA39, MECA39, PNAS 121, PP18, Protein ECA39

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67084-1-PBS targets BCAT1 in WB, Indirect ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28328

Product name: Recombinant human BCAT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-320 aa of BC033864

Sequence: MDCSNGCSAECTGEGGSKEVVGTFKAKDLIVTPATILKEKPDPNNLVFGTVFTDHMLTVEWSSEFGWEKPHIKPLQNLSLHPGSSALHYAVELFEGLKAFRGVDNKIRLFQPNLNMDRMYRSAVRATLPVFDKEELLECIQQLVKLDQEWVPYSTSASLYIRPTFIGTEPSLGVKKPTKALLFVLLSPVGPYFSSGTFNPVSLWANPKYVRAWKGGTGDCKMGGNYGSSLFAQCEAVDNGCQQVLWLYGEDHQITEVGTMNLFLYWINEDGEEELATPPLDGIILPGVTRRCILDLAHQWDTELSLFSINLPDFLQFIYF

相同性解析による交差性が予測される生物種
Full Name branched chain aminotransferase 1, cytosolic
Calculated molecular weight 320 aa, 36 kDa
Observed molecular weight 42-45 kDa
GenBank accession numberBC033864
Gene Symbol BCAT1
Gene ID (NCBI) 586
RRIDAB_2882392
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP54687
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BCAT1, also named as branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149).

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