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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BCAT1 Polyclonal antibody (13640-1-AP)

    WB analysis of HeLa using 13640-1-AP (same clone as 13640-1-PBS)

    WB result of BCAT1/ECA39 antibody (13640-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCAT1/ECA39 transfected HeLa cells. This data was developed using the same antibody clone with 13640-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BCAT1 Polyclonal antibody (13640-1-AP)

    WB analysis using 13640-1-AP (same clone as 13640-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13640-1-AP (BCAT1/ECA39 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13640-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BCAT1 Polyclonal antibody (13640-1-AP)

    IHC staining of mouse brain using 13640-1-AP (same clone as 13640-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13640-1-AP (BCAT1/ECA39 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13640-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using BCAT1 Polyclonal antibody (13640-1-AP)

    IHC staining of human stomach cancer using 13640-1-AP (same clone as 13640-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13640-1-AP (BCAT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13640-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BCAT1 Polyclonal antibody (13640-1-AP)

    IF Staining of HepG2 using 13640-1-AP (same clone as 13640-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BCAT1 antibody (13640-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13640-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

BCAT1 Polyclonal antibody, PBS Only

BCAT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13640-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PNAS 121, MECA39, ECA39, EC:2.6.1.42, Branched-chain-amino-acid aminotransferase, cytosolic

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13640-1-PBS targets BCAT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4574

Product name: Recombinant human BCAT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-320 aa of BC033864

Sequence: MDCSNGCSAECTGEGGSKEVVGTFKAKDLIVTPATILKEKPDPNNLVFGTVFTDHMLTVEWSSEFGWEKPHIKPLQNLSLHPGSSALHYAVELFEGLKAFRGVDNKIRLFQPNLNMDRMYRSAVRATLPVFDKEELLECIQQLVKLDQEWVPYSTSASLYIRPTFIGTEPSLGVKKPTKALLFVLLSPVGPYFSSGTFNPVSLWANPKYVRAWKGGTGDCKMGGNYGSSLFAQCEAVDNGCQQVLWLYGEDHQITEVGTMNLFLYWINEDGEEELATPPLDGIILPGVTRRCILDLAHQWDTELSLFSINLPDFLQFIYF

相同性解析による交差性が予測される生物種
Full Name branched chain aminotransferase 1, cytosolic
Calculated molecular weight 320 aa, 36 kDa
Observed molecular weight 43-45 kDa
GenBank accession numberBC033864
Gene Symbol BCAT1
Gene ID (NCBI) 586
RRIDAB_2063569
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP54687
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BCAT1, also named branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine, and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149). The 13640-1-AP antibody detects the bands around 43-45 kDa for different isoforms and higher molecular weight for phosphorylation modification of this protein.

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