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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BAP31 Recombinant monoclonal antibody (84584-5-RR)

    WB analysis using 84584-5-RR (same clone as 84584-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84584-5-RR (BAP31 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84584-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BAP31 Recombinant monoclonal antibody (84584-5-RR)

    IHC staining of human colon cancer using 84584-5-RR (same clone as 84584-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84584-5-RR (BAP31 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84584-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BAP31 Recombinant monoclonal antibody (84584-5-RR)

    IHC staining of human colon cancer using 84584-5-RR (same clone as 84584-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84584-5-RR (BAP31 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84584-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using BAP31 Recombinant monoclonal antibody (84584-5-RR)

    IHC staining of human placenta using 84584-5-RR (same clone as 84584-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84584-5-RR (BAP31 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84584-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using BAP31 Recombinant monoclonal antibody (84584-5-RR)

    IHC staining of human placenta using 84584-5-RR (same clone as 84584-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84584-5-RR (BAP31 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84584-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BAP31 Recombinant monoclonal antibody (84584-5-RR)

    IF Staining of HeLa using 84584-5-RR (same clone as 84584-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BAP31 antibody (84584-5-RR, Clone: 241978D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84584-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using BAP31 Recombinant monoclonal antibody (84584-5-RR)

    FC experiment of HepG2 using 84584-5-RR (same clone as 84584-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug BAP31 Recombinant antibody (84584-5-RR, Clone:241978D10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84584-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-3 cells using BAP31 Recombinant monoclonal antibody (84584-5-RR)

    FC experiment of PC-3 using 84584-5-RR (same clone as 84584-5-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug BAP31 Recombinant antibody (84584-5-RR, Clone:241978D10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84584-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84584-5-RR

    Affinity and Kinetic Characterization of 84584-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84584-5-RR against Human BAP31 were performed. The affinity constant is below 1 pM.

BAP31 Recombinant monoclonal antibody, PBS Only

BAP31 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84584-5-PBS
Clone No.241978D10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

BCAP31, 241978D10, 6C6 AG, 6C6-AG tumor-associated antigen, B-cell receptor-associated protein 31

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84584-5-PBS targets BAP31 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1668

Product name: Recombinant human BCAP31 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-246 aa of BC014323

Sequence: MSLQWTAVATFLYAEVFVVLLLCIPFISPKRWQKIFKSRLVELLVSYGNTFFVVLIVILVLLVIDAVREIREYDDVTEKVNLQNNPGAMEHFHMKLFRAQRNLYIAGFSLLLSFLLRRLVTLISQQATLLASNEAFKKQAESASEAAKKYMEENDQLKKGAAVDGGKLDVGNAEVKLEEENRSLKADLQKLKDELASTKQKLEKAENQVLAMRKQSEGLTKEYDRLLEEHAKLQAAVDGPMDKKEE

相同性解析による交差性が予測される生物種
Full Name B-cell receptor-associated protein 31
Calculated molecular weight 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC014323
Gene Symbol BAP31
Gene ID (NCBI) 10134
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP51572
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BAP31, also known as BCAP31, is a chaperone protein abundant in the endoplasmic reticulum (ER). BAP31 plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded proteins, and their targeting to the ER-associated-degradation (ERAD). CDIP1 and BAP31 interact upon ER stress to regulate the mitochondrial apoptosis pathway(PMID: 24139803). It also serves as a cargo receptor for the export of transmembrane proteins. BAP31 may be involved in apoptosis.

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