• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using BAP29 Polyclonal antibody (15796-1-AP)

    WB analysis using 15796-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15796-1-AP (BAP29 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using BAP29 Polyclonal antibody (15796-1-AP)

    WB analysis of COLO 320 using 15796-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 15796-1-AP (BAP29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using BAP29 Polyclonal antibody (15796-1-AP)

    IHC staining of human lymphoma using 15796-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 15796-1-AP (BAP29 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using BAP29 Polyclonal antibody (15796-1-AP)

    IHC staining of human lymphoma using 15796-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 15796-1-AP (BAP29 antibody) at dilution of 1:200 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BAP29 Polyclonal antibody (15796-1-AP)

    IF Staining of HeLa using 15796-1-AP

    Immunofluorescent analysis of Hela cells, using BCAP29 antibody 15796-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HEK-293 cells using BAP29 Polyclonal antibody (15796-1-AP)

    FC experiment of HEK-293 using 15796-1-AP

    1x10^6 HEK-293 cells were intracellularly stained with 0.5 ug BAP29 Polyclonal antibody (15796-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

BAP29 Polyclonal antibody

BAP29 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 15796-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

BCAP29, B-cell receptor-associated protein 29, BCR-associated protein 29

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, COLO 320 cells, Raji cells, U-87 MG cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15796-1-AP targets BAP29 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8505

Product name: Recombinant human BCAP29 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 118-241 aa of BC008478

Sequence: RRLVTLITQLAKELSNKGVLKTQAENTNKAAKKFMEENEKLKRILKSHGKDEECVLEAENKKLVEDQEKLKTELRKTSDALSKAQNDVMEMKMQSERLSKEYDQLLKEHSELQDRLERGNKKRL

相同性解析による交差性が予測される生物種
Full Name B-cell receptor-associated protein 29
Calculated molecular weight 241 aa, 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC008478
Gene Symbol BAP29
Gene ID (NCBI) 55973
RRIDAB_2065118
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHQ4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for BAP29 antibody 15796-1-APDownload protocol
IF protocol for BAP29 antibody 15796-1-APDownload protocol
IHC protocol for BAP29 antibody 15796-1-APDownload protocol
WB protocol for BAP29 antibody 15796-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

Legionella uses host Rab GTPases and BAP31 to create a unique ER niche

Authors - Attinder Chadha
View Article
||
New chat Able
正在加载，请稍候...