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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BCAM Polyclonal antibody

BCAM Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28222-1-AP

Synonyms

AU, Auberger B antigen, Basal cell adhesion molecule, BCAM, CD239, F8/G253 antigen, LU, Lutheran antigen, MSK19

Validation Data Gallery

Applications:
  • WB analysis using 28222-1-AP

    WB analysis using 28222-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28222-1-AP (BCAM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA431 cells, HT-29 cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

28222-1-AP targets BCAM in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCAM fusion protein Ag28181 相同性解析による交差性が予測される生物種
Full Name basal cell adhesion molecule (Lutheran blood group)
Calculated molecular weight 67 kDa
Observed molecular weight67-80 kDa
GenBank accession numberBC050450
Gene symbol BCAM
Gene ID (NCBI) 4059
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCAM (Lutheran/basal cell-adhesion molecule, a glycoprotein) belongs to the immunoglobulin superfamily which contains both Lu blood group and BCAM tumor-associated antigens. The significance of Lu/BCAM on tumorigenesis has been increasingly recognized. Lu/BCAM is involved in cell-cell adhesion and migration in skin tumor (PMID: 11121140, PMID: 10728810). Proteomics analysis suggests that BCAM might be a potential biomarker for pancreatic cancer (PMID: 19199705).

Protocols

Product Specific Protocols
WB protocol for BCAM antibody 28222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols