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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using BBS7 Polyclonal antibody (18961-1-AP)

    WB analysis of human brain using 18961-1-AP (same clone as 18961-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 18961-1-AP (BBS7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18961-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using BBS7 Polyclonal antibody (18961-1-AP)

    WB analysis of human brain using 18961-1-AP (same clone as 18961-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 18961-1-AP (BBS7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18961-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using BBS7 Polyclonal antibody (18961-1-AP)

    IP experiment of mouse testis using 18961-1-AP (same clone as 18961-1-PBS)

    IP result of anti-BBS7 (IP:18961-1-AP, 4ug; Detection:18961-1-AP 1:500) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 18961-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using BBS7 Polyclonal antibody (18961-1-AP)

    IHC staining of human testis using 18961-1-AP (same clone as 18961-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 18961-1-AP (BBS7 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18961-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using BBS7 Polyclonal antibody (18961-1-AP)

    IHC staining of human testis using 18961-1-AP (same clone as 18961-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 18961-1-AP (BBS7 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18961-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using BBS7 Polyclonal antibody (18961-1-AP)

    IHC staining of human brain using 18961-1-AP (same clone as 18961-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 18961-1-AP (BBS7 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18961-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using BBS7 Polyclonal antibody (18961-1-AP)

    IHC staining of human brain using 18961-1-AP (same clone as 18961-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 18961-1-AP (BBS7 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18961-1-PBS in a different storage buffer formulation.

BBS7 Polyclonal antibody, PBS Only

BBS7 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 18961-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18961-1-PBS targets BBS7 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4601

Product name: Recombinant human BBS7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC032691

Sequence: MDLILNRMDYLQVGVTSQKTMKLIPASRHRATQKVVIGDHDGVVMCFGMKKGEAAAVFKTLPGPKIARLELGGVINTPQEKIFIAAASEIRGFTKRGKQFLSFETNLTESIKAMHISGSDLFLSASYIYNHYCDCKDQHYYLSGDKINDVICLPVERLSRITPVLACQDRVLRVLQGSDVMYAVEVPGPPTVLALHNGNGGDSGEDLLFGTSDGKLALIQITTSKPVRKWEIQNEKKRGGILCIDSFDIVGDGVKDLLVGRDDGMVEVYSFDNANEPVLRFDQMLSESVTSIQGGCVGKDSYDEIVVSTYSGWVTGLTTEPIHKESGPGEELKINQEMQNKISSLRNELE

相同性解析による交差性が予測される生物種
Full Name Bardet-Biedl syndrome 7
Calculated molecular weight 715 aa, 80 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC032691
Gene Symbol BBS7
Gene ID (NCBI) 55212
RRIDAB_2064998
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWZ6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BBS7, also named as BBS2L1, is a component of the BBSome complex which is required for ciliogenesis but is dispensable for centriolar satellite function. BBS7 is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome.

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