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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using BBS6 Polyclonal antibody (13078-1-AP)

    WB analysis of mouse testis using 13078-1-AP (same clone as 13078-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13078-1-AP (BBS6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13078-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using BBS6 Polyclonal antibody (13078-1-AP)

    WB analysis of mouse brain using 13078-1-AP (same clone as 13078-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13078-1-AP (BBS6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13078-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using BBS6 Polyclonal antibody (13078-1-AP)

    IHC staining of mouse testis using 13078-1-AP (same clone as 13078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13078-1-AP (BBS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13078-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using BBS6 Polyclonal antibody (13078-1-AP)

    IHC staining of mouse testis using 13078-1-AP (same clone as 13078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13078-1-AP (BBS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13078-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using BBS6 Polyclonal antibody (13078-1-AP)

    IF Staining of hTERT-RPE1 cells using 13078-1-AP (same clone as 13078-1-PBS)

    IF result (cytoplasm and the base of cilia stain) of anti-BBS6 (13078-1-AP; 1:50) with hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim. This data was developed using the same antibody clone with 13078-1-PBS in a different storage buffer formulation.

BBS6 Polyclonal antibody, PBS Only

BBS6 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13078-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13078-1-PBS targets BBS6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3785

Product name: Recombinant human BBS6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 223-570 aa of BC028973

Sequence: SEVQLMRLLPIKKSTALKVALFCTTLSGDTSDTGEGTVVVSYGVSLENAVLDQLLNLGRQLISDHVDLVLCQKVIHPSLKQFLNMHRIIAIDRIGVTLMEPLTKMTGTQPIGSLGSICPNSYGSVKDVCTAKFGSKHFFHLIPNEATICSLLLCNRNDTAWDELKLTCQTALHVLQLTLKEPWALLGGGCTETHLAAYIRHKTHNDPESILKDDECTQTELQLIAEAFCSALESVVGSLEHDGGEILTDMKYGHLWSVQADSPCVANWPDLLSQCGCGLYNSQEELNWSFLRSTCRPFVPQSCLPHEAVVSASNLTLDCLTAKLSGLQVAVETANLILDLSYVIEDKN

相同性解析による交差性が予測される生物種
Full Name McKusick-Kaufman syndrome
Calculated molecular weight 62 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC028973
Gene Symbol MKKS
Gene ID (NCBI) 8195
RRIDAB_10603370
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NPJ1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MKKS also known as BBS6 is a probable chaperone given to the amino acid similarity to the chaperonin family of proteins and may play a role in protein processing in limb, cardiac and reproductive system development. The mutations in BBS6 have been linked to Bardet-Biedl syndrome (BBS) which is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. It may also get involved in cellular organization processes, in particular relating to ciliary/flagellar and centrosomal activities.

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