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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BBS4 Polyclonal antibody (12766-1-AP)

    WB analysis using 12766-1-AP (same clone as 12766-1-PBS)

    HEK-293 cell lysates were subjected to SDS PAGE followed by western blot with 12766-1-AP (BBS4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using BBS4 Polyclonal antibody (12766-1-AP)

    WB analysis of mouse kidney using 12766-1-AP (same clone as 12766-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12766-1-AP (BBS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using BBS4 Polyclonal antibody (12766-1-AP)

    IHC staining of mouse stomach using 12766-1-AP (same clone as 12766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12766-1-AP (BBS4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using BBS4 Polyclonal antibody (12766-1-AP)

    IHC staining of rat kidney using 12766-1-AP (same clone as 12766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12766-1-AP (BBS4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using BBS4 Polyclonal antibody (12766-1-AP)

    IF Staining of hTERT-RPE1 cells using 12766-1-AP (same clone as 12766-1-PBS)

    IF result (cytoplasm and the length of cilia cilia stain) of anti-BBS4 (12766-1-AP; 1:50) with serum-starved hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim. This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using BBS4 Polyclonal antibody (12766-1-AP)

    IF Staining of hTERT-RPE1 using 12766-1-AP (same clone as 12766-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using BBS4 antibody (12766-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

BBS4 Polyclonal antibody, PBS Only

BBS4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12766-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Bardet Biedl syndrome 4, Bardet-Biedl syndrome 4 protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12766-1-PBS targets BBS4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3430

Product name: Recombinant human BBS4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC027624

Sequence: MAEERVATRTQFPVSTESQKPRQKKAPEFPILEKQNWLIHLHYIRKDYEACKAVIKEQLQETQGLCEYAIYVQALIFRLEGNIQESLELFQTCAVLSPQSADNLKQVARSLFLLGKHKAAIEVYNEAAKLNQKDWEISHNLGVCYIYLKQFNKAQDQLHNALNLNRHDLTYIMLGKIHLLEGDLDKAIEVYKKAVEFSPENTELLTTLGLLYLQLGIYQKAFEHLGNALTYDPTNYKAILAAGSMMQTHGDFDVALTKYRVVACAVPESPPLWNNIGMCFFGKKKYVAAISCLKRANYLAPFDWKILYNLGLVHLTMQQYASAFHFLSAAINFQPKMGELYMLLAVALTNL

相同性解析による交差性が予測される生物種
Full Name Bardet-Biedl syndrome 4
Calculated molecular weight 519 aa, 58 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC027624
Gene Symbol BBS4
Gene ID (NCBI) 585
RRIDAB_10596774
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RK4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BBS4 (Bardet-Biedl syndrome protein 4) is one of the early BBS proteins discovered, with 35 mutations reported to cause the BBS phenotype (PMID: 26762677). BBS4 undergoes localization to the centriolar satellites of centrosomes and basal body of primary cilia, where it takes part in recruiting cargo to centriolar satellites and allowing the formation of a functional centrosomal microtubule organizing center (MTOC) (PMID: 25860617). Some research has found that BBS4 expression in humans during the development of CNS and sensory organs, concerning the cardinal clinical signs of BBS (PMID: 33860840).

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