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Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using BBS3 Polyclonal antibody (12676-1-AP)

    WB analysis of rat brain using 12676-1-AP (same clone as 12676-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 12676-1-AP (BBS3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using BBS3 Polyclonal antibody (12676-1-AP)

    WB analysis of mouse brain using 12676-1-AP (same clone as 12676-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12676-1-AP (BBS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using BBS3 Polyclonal antibody (12676-1-AP)

    WB analysis of human brain using 12676-1-AP (same clone as 12676-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12676-1-AP (BBS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293T cells using BBS3 Polyclonal antibody (12676-1-AP)

    WB analysis of HEK-293T using 12676-1-AP (same clone as 12676-1-PBS)

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 12676-1-AP (BBS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of rat brain tissue using BBS3 Polyclonal antibody (12676-1-AP)

    IP experiment of rat brain using 12676-1-AP (same clone as 12676-1-PBS)

    IP result of anti-BBS3 (IP:12676-1-AP, 3ug; Detection:12676-1-AP 1:600) with rat brain tissue lysate 5200ug. This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using BBS3 Polyclonal antibody (12676-1-AP)

    IHC staining of human kidney using 12676-1-AP (same clone as 12676-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12676-1-AP (BBS3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using BBS3 Polyclonal antibody (12676-1-AP)

    IHC staining of human kidney using 12676-1-AP (same clone as 12676-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12676-1-AP (BBS3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using BBS3 Polyclonal antibody (12676-1-AP)

    IF Staining of hTERT-RPE1 cells using 12676-1-AP (same clone as 12676-1-PBS)

    IF result (cytoplasm and the base of cilia stain) of anti-BBS3 (12676-1-AP; 1:50) with hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim. This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using BBS3 Polyclonal antibody (12676-1-AP)

    IF Staining of MDCK using 12676-1-AP (same clone as 12676-1-PBS)

    Immunofluorescent analysis of MDCK cells using 12676-1-AP (BBS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

BBS3 Polyclonal antibody, PBS Only

BBS3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12676-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12676-1-PBS targets BBS3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3363

Product name: Recombinant human BBS3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-186 aa of BC024239

Sequence: MGLLDRLSVLLGLKKKEVHVLCLGLDNSGKTTIINKLKPSNAQSQNILPTIGFSIEKFKSSSLSFTVFDMSGQGRYRNLWEHYYKEGQAIIFVIDSSDRLRMVVAKEELDTLLNHPDIKHRRIPILFFANKMDLRDAVTSVKVSQLLCLENIKDKPWHICASDAIKGEGLQEGVDWLQDQIQTVKT

相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor-like 6
Calculated molecular weight 186 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC024239
Gene Symbol ARL6/BBS3
Gene ID (NCBI) 84100
RRIDAB_2058675
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0F7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ADP-ribosylation factor-like protein 6 (ARL6), belongs to a small GTPase superfamily, is involved in membrane protein trafficking at the base of the ciliary organelle. ARL6 is also named Bardet-Biedl syndrome 3 protein, because defects in ARL6 are a cause of Bardet-Biedl syndrome type 3 (BBS3), which is a genetically heterogeneous disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. ARL6 can interact with many ARL6 interacting proteins and form BBSome complex with other BBS proteins including BBS1, BBS2, BBS4 and so on.

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