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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MKS1 Monoclonal antibody (66518-1-Ig)

    WB analysis using 66518-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66518-1-Ig (BBS13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using MKS1 Monoclonal antibody (66518-1-Ig)

    WB analysis of pig brain using 66518-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66518-1-Ig (BBS13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MKS1 Monoclonal antibody (66518-1-Ig)

    IHC staining of human liver cancer using 66518-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MKS1 Monoclonal antibody (66518-1-Ig)

    IHC staining of mouse brain using 66518-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MKS1 Monoclonal antibody (66518-1-Ig)

    IHC staining of mouse brain using 66518-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MKS1 Monoclonal antibody (66518-1-Ig)

    IHC staining of human liver cancer using 66518-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MKS1 Monoclonal antibody

MKS1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66518-1-Ig
Clone No.6A8B11

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat, mouse

Applications

WB, IHC, ELISA

Synonyms

BBS13, Meckel syndrome type 1 protein, Meckel syndrome, type 1, MES, MKS, MKS1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNCCIT cells, pig brain tissue, HEK-293 cells, HSC-T6 cells
Positive IHC detected inmouse brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66518-1-Ig targets MKS1 in WB, IHC, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

Tested Reactivity Human, pig, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag9504

Product name: Recombinant human BBS13 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-321 aa of BC010061

Sequence: MTTAASEVPSFLVERMANVRRRRQDRRGMEGGILKSRIVTWEPSEEFVRNNHVINTPLQTMHIMADLGPYKKLGYKKYEHVLCTLKVDSNGVITVKPDFTGLKGPYRIETEGEKQELWKYTIDNVSPHAQPEEEERERRVFKDLYGRHKEYLSSLVGTDFEMTVPGALRLFVNGEVVSAQGYEYDNLYVHFFVELPTAHWSSPAFQQLSGVTQTCTTKSLAMDKVAHFSYPFTFEAFFLHEDESSDALPEWPVLYCEVLSLDFWQRYRVEGYGAVVLPATPGSHTLTVSTWRPVELGTVAELRRFFIGGSLELEDLSYVRI

相同性解析による交差性が予測される生物種
Full Name Meckel syndrome, type 1
Calculated molecular weight 559 aa, 65 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC010061
Gene Symbol MKS1
Gene ID (NCBI) 54903
RRIDAB_2881881
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9NXB0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MKS1 antibody 66518-1-IgDownload protocol
IHC protocol for MKS1 antibody 66518-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols