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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using BBS10 Polyclonal antibody (12421-2-AP)

    WB analysis of HepG2 using 12421-2-AP (same clone as 12421-2-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12421-2-AP (BBS10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12421-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using BBS10 Polyclonal antibody (12421-2-AP)

    WB analysis of A549 using 12421-2-AP (same clone as 12421-2-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 12421-2-AP (BBS10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12421-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using BBS10 Polyclonal antibody (12421-2-AP)

    WB analysis of MCF-7 using 12421-2-AP (same clone as 12421-2-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12421-2-AP (BBS10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12421-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using BBS10 Polyclonal antibody (12421-2-AP)

    IF Staining of hTERT-RPE1 cells using 12421-2-AP (same clone as 12421-2-PBS)

    IF result (nuclear and the base of cilia stain) of anti-BBS10 (12421-2-AP; 1:50) with hTERT-RPE1 cell (PFA fixed) by Dr. Moshe Kim. This data was developed using the same antibody clone with 12421-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

BBS10 Polyclonal antibody, PBS Only

BBS10 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 12421-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12421-2-PBS targets BBS10 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3073

Product name: Recombinant human BBS10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 573-723 aa of BC013795

Sequence: MLPVSCKLPNMGTSQSYLSSSMPAGCVLPVGGNFEILLHYYLLNYAKKCHQSEETMVSMIIANALLGIPKVLYKSKTGKYSFPHTYIRAVHALQTNQPLVSSQTGLESVMGKYQLLTSVLQCLTKILTIDMVITVKRHPQKVHNQDSEDEL

相同性解析による交差性が予測される生物種
Full Name Bardet-Biedl syndrome 10
Calculated molecular weight 81 kDa
Observed molecular weight 81 kDa
GenBank accession numberBC013795
Gene Symbol BBS10
Gene ID (NCBI) 79738
RRIDAB_2064962
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TAM1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BBS10, also named as C12orf58, belongs to the TCP-1 chaperonin family. It is probable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, BBS10 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation. (PMID:20080638,19190184) Defects in BBS10 are the cause of Bardet-Biedl syndrome type 10 (BBS10).

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