Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, PANC-1 cells, U-87 MG cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33050-1-AP targets BBIP1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36924 Product name: Recombinant human BBIP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-92 aa of BC015550 Sequence: MLKAAAKRPELSGKNTISNNSDMAEVKSMFREVLPKQGPLFVEDIMTMVLCKPKLLPLKSLTLEKLEKMHQAAQNTIRQQEMAEKDQRQITH* 相同性解析による交差性が予測される生物種 |
| Full Name | non-protein coding RNA 81 |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC015550 |
| Gene Symbol | NCRNA00081 |
| Gene ID (NCBI) | 92482 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | A8MTZ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The BBIP1 protein is a key component of the BBSome complex and was identified due to its role in the pathogenesis of Bardet-Biedl syndrome (BBS). This protein localizes to the base of cellular cilia and plays an indispensable role in ciliary assembly, maintenance, and signal transduction. As an adapter for membrane protein trafficking, BBIP1 works in coordination with other members of the BBSome to precisely transport specific cargo proteins into the cilium, an important cellular organelle. Loss of its function disrupts the stability of the BBSome complex, leading to abnormalities in ciliary structure and function. This, in turn, causes various clinical symptoms of BBS, including retinal degeneration, obesity, and polydactyly. Therefore, research on BBIP1 is central to understanding the molecular basis of cilia-related diseases (ciliopathies).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BBIP1 antibody 33050-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |