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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BAT5 Polyclonal antibody (17317-1-AP)

    WB analysis using 17317-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17317-1-AP (BAT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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BAT5 Polyclonal antibody

BAT5 Polyclonal Antibody for WB, ELISA
Cat No. 17317-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

ABHD16A, Alpha/beta hydrolase domain-containing protein 16A, EC:3.1.-.-, EC:3.1.1.23, G5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue, mouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17317-1-AP targets BAT5 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAT5 fusion protein Ag11287 相同性解析による交差性が予測される生物種
Full Name HLA-B associated transcript 5
Calculated molecular weight 558 aa, 63 kDa
Observed molecular weight59~63 KdA
GenBank accession numberBC031839
Gene Symbol BAT5
Gene ID (NCBI) 7920
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95870
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human lymphocyte antigen B-associated transcript 5 (BAT5, also known as ABHD16A) is a 558 residue (63 kDa) protein encoded by 20 exons located on chromosome 6p21.33. BAT5 belongs to the α/β-hydrolase domain (ABHD) containing family of metabolic serine hydrolases and possesses both an esterase catalytic triad and an acyltransferase domain (PMID: 25290914). BAT5 not only participates in lipid metabolism but is also involved in the regulation of inflammation and immunity. BAT5 is predicted to be an integral membrane protein with highest mRNA transcript levels in mouse tissues found in testis, heart, muscle, and brain (PMID: 23328280).

Protocols

Product Specific Protocols
WB protocol for BAT5 antibody 17317-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols