Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31236-1-AP targets BANF2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35307 Product name: Recombinant human BANF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-90 aa of PL00285355 Sequence: MDNMSPRLRAFLSEPIGEKDVCWVDGISHELAINLVTKGINKAYILLGQFLLMHKNEAEFQRWLICCFGATECEAQQTSHCLKEWCACFL 相同性解析による交差性が予測される生物種 |
| Full Name | barrier to autointegration factor 2 |
| Calculated molecular weight | 10 kDa |
| Observed molecular weight | 11 kDa |
| GenBank accession number | PL00285355 |
| Gene Symbol | BANF2 |
| Gene ID (NCBI) | 140836 |
| RRID | AB_3669913 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9H503 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BANF2 (Barrier-to-autointegration factor 2), also called BAF-L (Barrier-to-autointegration factor-like protein), is a homologous gene termed BAF, which shows a 53% similarity in the protein sequence with BANF2 in humans (PMID: 35216101; 16337940). Unlike BAF, BANF2 plays an important role in spermiogenesis and spermatozoal function, and it is a male germ cell marker, whose expression is highly associated with the maturation of male germ cells (PMID: 33951625; 35216101).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BANF2 antibody 31236-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |