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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BANF1 Polyclonal antibody (30525-1-AP)

    WB analysis of HeLa using 30525-1-AP (same clone as 30525-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 30525-1-AP (BANF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30525-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BANF1 Polyclonal antibody (30525-1-AP)

    IF Staining of HepG2 using 30525-1-AP (same clone as 30525-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BANF1 antibody (30525-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30525-1-PBS in a different storage buffer formulation.

BANF1 Polyclonal antibody, PBS Only

BANF1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30525-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

D14S1460, BAF

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30525-1-PBS targets BANF1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag32042

Product name: Recombinant human BANF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 50-89 aa of BC005942

Sequence: LVLKKDEDLFR EWLKDTCGAN AKQSRDCFGC LREWCDAFL

相同性解析による交差性が予測される生物種
Full Name barrier to autointegration factor 1
Calculated molecular weight 10 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC005942
Gene Symbol BANF1
Gene ID (NCBI) 8815
RRIDAB_3086349
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75531
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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