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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using BAHCC1 Polyclonal antibody (30046-1-AP)

    IHC staining of mouse brain using 30046-1-AP (same clone as 30046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30046-1-AP (BAHCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30046-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BAHCC1 Polyclonal antibody (30046-1-AP)

    IHC staining of mouse brain using 30046-1-AP (same clone as 30046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30046-1-AP (BAHCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30046-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BAHCC1 Polyclonal antibody (30046-1-AP)

    IHC staining of mouse brain using 30046-1-AP (same clone as 30046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30046-1-AP (BAHCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30046-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using BAHCC1 Polyclonal antibody (30046-1-AP)

    IHC staining of rat brain using 30046-1-AP (same clone as 30046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30046-1-AP (BAHCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30046-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using BAHCC1 Polyclonal antibody (30046-1-AP)

    IHC staining of rat brain using 30046-1-AP (same clone as 30046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30046-1-AP (BAHCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30046-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using BAHCC1 Polyclonal antibody (30046-1-AP)

    IHC staining of mouse colon using 30046-1-AP (same clone as 30046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30046-1-AP (BAHCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30046-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BAHCC1 Polyclonal antibody (30046-1-AP)

    IHC staining of mouse brain using 30046-1-AP (same clone as 30046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30046-1-AP (BAHCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30046-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using BAHCC1 Polyclonal antibody (30046-1-AP)

    IF Staining of A549 using 30046-1-AP (same clone as 30046-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using BAHCC1 antibody (30046-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 30046-1-PBS in a different storage buffer formulation.

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BAHCC1 Polyclonal antibody, PBS Only

BAHCC1 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 30046-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30046-1-PBS targets BAHCC1 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAHCC1 fusion protein Ag32599 相同性解析による交差性が予測される生物種
Full Name BAH domain and coiled-coil containing 1
Calculated molecular weight280 kDa
Observed molecular weight 280 kDa
GenBank accession numberNM_001291324.1
Gene Symbol BAHCC1
Gene ID (NCBI) 57597
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P281
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BAHCC1 (BAH and coiled-coil domain-containing protein 1) is also named as BAHD2, KIAA1447 and BAH domain-containing protein 2. BAHCC1 is a transcriptional regulator controlling expression of E2F/KLF-dependent cell-cycle and DNA-repair genes. BAHCC1 associates with BRG1-containing remodeling complexes at the promoters of these genes. BAHCC1 silencing leads to decreased cell proliferation and delayed DNA repair. Consequently, BAHCC1 deficiency cooperates with PARP inhibition to induce melanoma cell death (PMID: 37924516). depletion of BAHCC1, or disruption of the BAHCC1BAH-H3K27me3 interaction, causes derepression of H3K27me3-targeted genes that are involved in tumor suppression and cell differentiation, leading to suppression of oncogenesis (PMID: 33139953).