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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BAG5 Recombinant antibody (86248-1-RR)

    WB analysis using 86248-1-RR (same clone as 86248-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86248-1-RR (BAG5 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86248-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using BAG5 Recombinant antibody (86248-1-RR)

    IF Staining of MCF-7 using 86248-1-RR (same clone as 86248-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using BAG5 antibody (86248-1-RR, Clone: 250877D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86248-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86248-1-RR

    Affinity and Kinetic Characterization of 86248-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86248-1-RR against Human BAG5 were performed. The affinity constant is 0.286 nM.

BAG5 Recombinant monoclonal antibody, PBS Only

BAG5 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86248-1-PBS
Clone No.250877D12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

BAG 5, BAG-5, Bcl-2-associated athanogene 5, KIAA0873

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86248-1-PBS targets BAG5 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag24320

Product name: Recombinant human BAG5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 46-394 aa of BC044216

Sequence: NQHPSISRLQEIQKEVKSVEQQVIGFSGLSDDKNYKKLERILTKQLFEIDSVDTEGKGDIQQARKRAAQETERLLKELEQNANHPHRIEIQNIFEEAQSLVREKIVPFYNGGNCVTDEFEEGIQDIILRLTHVKTGGKISLRKARYHTLTKICAVQEIIEDCMKKQPSLPLSEDAHPSVAKINFVMCEVNKARGVLIALLMGVNNNETCRHLSCVLSGLIADLDALDVCGRTEIRNYRREVVEDINKLLKYLDLEEEADTTKAFDLRQNHSILKIEKVLKRMREIKNELLQAQNPSELYLSSKTELQGLIGQLDEVSLEKNPCIREARRRAVIEVQTLITYIDLKEALE

相同性解析による交差性が予測される生物種
Full Name BCL2-associated athanogene 5
Calculated molecular weight 51 kDa
Observed molecular weight 48-51 kDa
GenBank accession numberBC044216
Gene Symbol BAG5
Gene ID (NCBI) 9529
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9UL15
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.