- Featured Product
- KD/KO Validated
BAG5 Polyclonal antibody
BAG5 Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 26628-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | MCF-7 cells, SH-SY5Y cells, mouse brain, rat brain |
| Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy BAG5 Ready-To-Use IHC Kit. BAG5 primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 1 publications below |
Product Information
26628-1-AP targets BAG5 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BAG5 fusion protein Ag24320 相同性解析による交差性が予測される生物種 |
| Full Name | BCL2-associated athanogene 5 |
| Calculated molecular weight | 51 kDa |
| Observed molecular weight | 48-51 kDa |
| GenBank accession number | BC044216 |
| Gene symbol | BAG5 |
| Gene ID (NCBI) | 9529 |
| RRID | AB_2880578 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BAG5 antibody 26628-1-AP | Download protocol |
| IHC protocol for BAG5 antibody 26628-1-AP | Download protocol |
| IF protocol for BAG5 antibody 26628-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Discov Neuromodulation of BAG co-chaperones by HIV-1 viral proteins and H2O2: implications for HIV-associated neurological disorders. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH WEI (Verified Customer) (03-03-2022) | It have three bands, one band is missing in the KO tissue.
|
