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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BAG3 Polyclonal antibody (10599-1-AP)

    WB analysis using 10599-1-AP (same clone as 10599-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BAG3 Polyclonal antibody (10599-1-AP)

    WB analysis using 10599-1-AP (same clone as 10599-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using BAG3 Polyclonal antibody (10599-1-AP)

    IP experiment of K-562 using 10599-1-AP (same clone as 10599-1-PBS)

    IP result of anti-BAG3 (IP:10599-1-AP, 4ug; Detection:10599-1-AP 1:1000) with K-562 cells lysate 11000ug. This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using BAG3 Polyclonal antibody (10599-1-AP)

    IHC staining of human gliomas using 10599-1-AP (same clone as 10599-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10599-1-AP (BAG3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using BAG3 Polyclonal antibody (10599-1-AP)

    IHC staining of human lung cancer using 10599-1-AP (same clone as 10599-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10599-1-AP (BAG3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using BAG3 Polyclonal antibody (10599-1-AP)

    IF Staining of A549 using 10599-1-AP (same clone as 10599-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using BAG3 antibody (10599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BAG3 Polyclonal antibody (10599-1-AP)

    IF Staining of HepG2 using 10599-1-AP (same clone as 10599-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BAG3 antibody (10599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BAG3 Polyclonal antibody (10599-1-AP)

    IF Staining of HeLa using 10599-1-AP (same clone as 10599-1-PBS)

    Immunofluorescent analysis of Hela cells, using BAG3 antibody 10599-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

BAG3 Polyclonal antibody, PBS Only

BAG3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10599-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

BAG-3, Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis, BIS, Docking protein CAIR-1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10599-1-PBS targets BAG3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAG3 fusion protein Ag0956 相同性解析による交差性が予測される生物種
Full Name BCL2-associated athanogene 3
Calculated molecular weight 61 kDa
Observed molecular weight 74-80 kDa
GenBank accession numberBC006418
Gene Symbol BAG3
Gene ID (NCBI) 9531
RRIDAB_2062602
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95817
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy. BAG3 normally migrates around 74-80 kDa; a slightly different molecular weight or a doublet form can be observed in some cell types and/or following cell exposure to stressors. A synaptosome associated form of 40 kDa has recently been described.