Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, U-251 cells, U-87 MG cells, mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
29820-1-AP targets BAG2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BAG2 fusion protein Ag30873 相同性解析による交差性が予測される生物種 |
| Full Name | BCL2-associated athanogene 2 |
| Calculated molecular weight | 24 kDa |
| Observed molecular weight | 25-27 kDa |
| GenBank accession number | NM_004282 |
| Gene Symbol | BAG2 |
| Gene ID (NCBI) | 9532 |
| RRID | AB_2918348 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95816 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BAG2 (BAG family molecular chaperone regulator 2) is one of six proteins in mammals that contain the BAG domain, which belongs to the BAG (Bcl-2-associated athanogene) family. BAG2 has been described as a negative regulator of the chaperone-associated ubiquitin ligase C terminus of Hsc70-interacting protein (CHIP) that participates in the ubiquitin-mediated proteasomal degradation of misfolded substrate proteins (PMID: 16207813). BAG2 is widely expressed in human tissues, including brown adipose, heart and lung tissue, as well as in various types of tumor cells, including renal cell carcinoma, glioblastoma and thyroid carcinoma cells (PMID: 28536620). BAG2 overexpression is associated with poor prognosis in patients and mutp53 accumulation in tumors (PMID: 26271008).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BAG2 antibody 29820-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
iScience A proximity labeling strategy enables proteomic analysis of inter-organelle membrane contacts |