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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BAD Monoclonal antibody (67830-1-Ig)

    WB analysis using 67830-1-Ig (same clone as 67830-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67830-1-Ig (BAD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67830-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using BAD Monoclonal antibody (67830-1-Ig)

    WB analysis of RAW 264.7 using 67830-1-Ig (same clone as 67830-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67830-1-Ig (BAD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67830-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BAD Monoclonal antibody (67830-1-Ig)

    IF Staining of HeLa using 67830-1-Ig (same clone as 67830-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BAD antibody (67830-1-Ig, Clone: 4B12G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67830-1-PBS in a different storage buffer formulation.

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BAD Monoclonal antibody, PBS Only

BAD Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67830-1-PBS
Clone No.4B12G2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

4B12G2, BBC2, BBC6, Bcl2-associated agonist of cell death, Bcl-2-binding component 6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67830-1-PBS targets BAD in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name BCL2-associated agonist of cell death
Calculated molecular weight 18 kDa
Observed molecular weight18-25 kDa
GenBank accession numberBC001901
Gene Symbol BAD
Gene ID (NCBI) 572
RRIDAB_2918592
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92934
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BAD, also named as BBC6 and BCL2L8, promotes cell death. It is successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. It appears to act as a link between growth factor receptor signaling and the apoptotic pathways.