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Target

Host

Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

BACH2 Polyclonal antibody

BACH2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27635-1-AP

Synonyms

BACH2, BTB and CNC homolog 2

Validation Data Gallery

Applications:
  • WB analysis of Daudi using 27635-1-AP

    WB analysis of Daudi using 27635-1-AP

    Daudi cells were subjected to SDS PAGE followed by western blot with 27635-1-AP (BACH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of Ramos using 27635-1-AP

    WB analysis of Ramos using 27635-1-AP

    Ramos cells were subjected to SDS PAGE followed by western blot with 27635-1-AP (BACH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of Daudi using 27635-1-AP

    WB analysis of Daudi using 27635-1-AP

    Daudi cells were subjected to SDS PAGE followed by western blot with 27635-1-AP (BACH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inDaudi cells, Ramos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

27635-1-AP targets BACH2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BACH2 fusion protein Ag26526 相同性解析による交差性が予測される生物種
Full Name BTB and CNC homology 1, basic leucine zipper transcription factor 2
Calculated molecular weight 93 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberNM_001170794
Gene symbol BACH2
Gene ID (NCBI) 60468
RRIDAB_2880934
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BACH2, also named as Transcription regulator protein BACH2, is a 841 amino acid protein, which is widely expressed within the B-lymphoid lineage, except in plasma cells. BACH2 contains an N-terminal BTB domain involved in its transcriptional function, but instead of having zinc fingers at the C-terminus, it binds to DNA through a basic leucine zipper motif. BACH2 binds DNA consensus sequences, termed MARE motifs, by forming a heterodimer with small leucine zipper MAF proteins such as MAFK, MAFG, and MAFF. The calculated molecular weight of BACH2 is 92 kDa, but the post-modifiction of BACH2 is about 120-130 kDa. (PMID: 24277074 )

Protocols

Product Specific Protocols
WB protocol for BACH2 antibody 27635-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

FEBS Open Bio

Bach2 overexpression represses Th9 cell differentiation by suppressing IRF4 expression in systemic lupus erythematosus.

Authors - Yujun Sheng
  • KD Validated
View Article