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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated BACH1 Monoclonal antibody

BACH1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, mouse, pig, rabbit

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D12A4

Cat no : CL594-66762

Synonyms

BACH 1, BACH1, BTB and CNC homolog 1, HA2303

Validation Data Gallery

Applications:
  • FC experiment of K-562 using CL594-66762

    FC experiment of K-562 using CL594-66762

    1x10^6 K-562 cells were intracellularly stained with 0.8 ug CoraLite®594-conjugated BACH1 Monoclonal antibody (CL594-66762, Clone:2D12A4)(red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inK-562 cells
For other applications, we recommend the unconjugated version of this antibody, 66762-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-66762 targets BACH1 in FC (Intra) applications and shows reactivity with Human, Rat, mouse, pig, rabbit samples.

Tested Reactivity Human, Rat, mouse, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BACH1 fusion protein Ag6011 相同性解析による交差性が予測される生物種
Full Name BTB and CNC homology 1, basic leucine zipper transcription factor 1
Calculated molecular weight 82 kDa
Observed molecular weight 100-110 kDa, 82 kDa
GenBank accession numberBC063307
Gene symbol BACH1
Gene ID (NCBI) 571
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BTB and CNC homology 1, basic leucine zipper transcription factor 1(BACH1) is a heme-regulated transcriptional regulator that acts as repressor or activator and a member of the AP-a superfamily. MafK belongs to the small Maf family that can funciton as transcriptional activators or repressors depending on the proteins with which they form heterdimers. BACH1 has crucial roles in both stress responses and transformation by H-RasV12. The molecular weight of expected mobility Bach1 is 100-110 kDa.

Protocols

Product Specific Protocols
FC protocol for CL594 BACH1 antibody CL594-66762Download protocol
Standard Protocols
Click here to view our Standard Protocols