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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using BACE1 Polyclonal antibody (12807-1-AP)

    WB analysis of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BACE1 Polyclonal antibody (12807-1-AP)

    WB analysis using 12807-1-AP (same clone as 12807-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using BACE1 Polyclonal antibody (12807-1-AP)

    WB analysis of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of zebrafish tissue using BACE1 Polyclonal antibody (12807-1-AP)

    WB analysis of zebrafish using 12807-1-AP (same clone as 12807-1-PBS)

    zebrafish tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using BACE1 Polyclonal antibody (12807-1-AP)

    WB analysis of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using BACE1 Polyclonal antibody (12807-1-AP)

    WB analysis of human brain using 12807-1-AP (same clone as 12807-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse pancreas tissue using BACE1 Polyclonal antibody (12807-1-AP)

    WB analysis of mouse pancreas using 12807-1-AP (same clone as 12807-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using BACE1 Polyclonal antibody (12807-1-AP)

    WB analysis of HeLa using 12807-1-AP (same clone as 12807-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using BACE1 Polyclonal antibody (12807-1-AP)

    IP experiment of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

    IP result of anti-BACE1 (IP:12807-1-AP, 3ug; Detection:12807-1-AP 1:500) with mouse brain tissue lysate 5000ug. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment using BACE1 Polyclonal antibody (12807-1-AP)

    IP experiment using 12807-1-AP (same clone as 12807-1-PBS)

    IP result of BACE1 antibody (12807-1-AP for IP and Detection) with mouse brain tissue lysate. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BACE1 Polyclonal antibody (12807-1-AP)

    IHC staining of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BACE1 Polyclonal antibody (12807-1-AP)

    IHC staining of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BACE1 Polyclonal antibody (12807-1-AP)

    IHC staining of human pancreas cancer using 12807-1-AP (same clone as 12807-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BACE1 Polyclonal antibody (12807-1-AP)

    IHC staining of human pancreas cancer using 12807-1-AP (same clone as 12807-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BACE1 Polyclonal antibody (12807-1-AP)

    IHC staining of human pancreas cancer using 12807-1-AP (same clone as 12807-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BACE1 Polyclonal antibody (12807-1-AP)

    IHC staining of human pancreas cancer using 12807-1-AP (same clone as 12807-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using BACE1 Polyclonal antibody (12807-1-AP)

    IF Staining of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BACE1 antibody (12807-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

BACE1 Polyclonal antibody, PBS Only

BACE1 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 12807-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12807-1-PBS targets BACE1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3559

Product name: Recombinant human BACE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 157-457 aa of BC036084

Sequence: VRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLM

相同性解析による交差性が予測される生物種
Full Name beta-site APP-cleaving enzyme 1
Calculated molecular weight 501 aa, 56 kDa
Observed molecular weight 70-75 kDa, 42-56 kDa
GenBank accession numberBC036084
Gene Symbol BACE1
Gene ID (NCBI) 23621
RRIDAB_10638760
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP56817
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

beta-secretase, encoded by BACE1(beta-stie amyloid beta A4 precursor protein-cleavage enzyem1) gene, is a neuron-specific membrane-associated protease that participate in the two step proteolytic processing of the APP. Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has a high expression in the brain and pancreas. This protein has 6 isoforms produced by alternative splicing. BACE1 can be detected with different molecular weight: 42-56kD (prozymogen and isoforms), 65-75 (glycosylated forms) and 160kDa (dimer) (PMID:22741101; 21795680).

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