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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using B7-H6 Polyclonal antibody (31620-1-AP)

    WB analysis using 31620-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31620-1-AP (B7-H6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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B7-H6 Polyclonal antibody

B7-H6 Polyclonal Antibody for WB, ELISA
Cat No. 31620-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

B7H6, NCR3LG1, Natural cytotoxicity triggering receptor 3 ligand 1, B7 homolog 6, B7 H6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31620-1-AP targets B7-H6 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name hypothetical protein DKFZp686O24166
Calculated molecular weight51 kDa
Observed molecular weight80 kDa
GenBank accession numberBC136800
Gene Symbol B7H6
Gene ID (NCBI) 374383
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ68D85
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

B7-H6, also known as NCR3LG1, is a member of the B7 family of immunoreceptors. It is a 454-aa-long type I transmembrane protein consisting of an extracellular region, a transmembrane segment, and a cytoplasmic domain which contains an immunoreceptor tyrosine-based inhibitory motif (ITIM), an SH2-binding domain, and an SH3-binding motif. B7-H6 triggers NKp30-mediated activation of human NK cells (PMID: 19528259). It has been reported that B7H6 is expressed in various types of tumor cells and plays a prominent role in natural killer cell immunosurveillance (PMID: 35697295). The apparent molecular weight of B7-H6 is larger than the calculated molecular weight of 51 kDa due to glycosylation (PMID: 19528259).

Protocols

Product Specific Protocols
WB protocol for B7-H6 antibody 31620-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols