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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

B7-H4 Recombinant antibody

B7-H4 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4C8

Cat no : 82850-1-RR

Synonyms

B7 H4, B7h.5, B7H4, B7S1, B7X, FLJ22418, PRO1291, Protein B7S1, RP11 229A19.4, VCTN1, VTCN1

Validation Data Gallery

Applications:
  • WB analysis using 82850-1-RR

    WB analysis using 82850-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82850-1-RR (B7‑H4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, SKOV-3 cells, BGC-823 cells, SK-BR-3 cells
Planning an IHC experiment? We recommend our IHCeasy VTCN1 Ready-To-Use IHC Kit. VTCN1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Sample-dependent, check data in validation data gallery

Product Information

82850-1-RR targets B7-H4 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name V-set domain containing T cell activation inhibitor 1
Calculated molecular weight282 aa, 31 kDa
Observed molecular weight75 kDa
GenBank accession numberNM_024626
Gene symbol VTCN1
Gene ID (NCBI) 79679
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

B7-H4, also named VTCN1, B7X, or B7S1, is a single-pass type I membrane protein, which contains two immunoglobulin-like domains and belongs to the immunoglobulin superfamily. B7-H4 negatively regulates T-cell mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. B7-H4 is over-expressed in many cancers, including breast, ovarian, endometrial, renal cell and non-small-cell lung cancers. The predicted molecular weight of B7‑H4 is 31 kDa. The glycosylated B7‑H4 is 50 to 80 kDa, and the non-glycosylated form is 28 kDa.

Protocols

Product Specific Protocols
WB protocol for B7-H4 antibody 82850-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols