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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using B7‑H4 Polyclonal antibody (12080-1-AP)

    WB analysis of mouse liver using 12080-1-AP (same clone as 12080-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12080-1-AP (B7‑H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using B7‑H4 Polyclonal antibody (12080-1-AP)

    WB analysis of A549 using 12080-1-AP (same clone as 12080-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 12080-1-AP (B7‑H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using B7‑H4 Polyclonal antibody (12080-1-AP)

    WB analysis of rat liver using 12080-1-AP (same clone as 12080-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 12080-1-AP (B7‑H4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using B7‑H4 Polyclonal antibody (12080-1-AP)

    IHC staining of human ovary tumor using 12080-1-AP (same clone as 12080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12080-1-AP (B7‑H4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using B7‑H4 Polyclonal antibody (12080-1-AP)

    IHC staining of human breast cancer using 12080-1-AP (same clone as 12080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12080-1-AP (B7‑H4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using B7‑H4 Polyclonal antibody (12080-1-AP)

    IHC staining of human breast cancer using 12080-1-AP (same clone as 12080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12080-1-AP (B7‑H4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using B7‑H4 Polyclonal antibody (12080-1-AP)

    IHC staining of human ovary tumor using 12080-1-AP (same clone as 12080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12080-1-AP (B7‑H4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12080-1-PBS targets B7‑H4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B7‑H4 fusion protein Ag2712 相同性解析による交差性が予測される生物種
Full Name V-set domain containing T cell activation inhibitor 1
Calculated molecular weight 282 aa, 31 kDa
Observed molecular weight 55-65 kDa
GenBank accession numberBC065717
Gene Symbol B7-H4
Gene ID (NCBI) 79679
RRIDAB_2877822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z7D3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

B7‑H4 also named VTCN1, B7X, or B7S1 is a 282 amino acid protein, which contains 2 immunoglobulin-like domains and belongs to the immunoglobulin superfamily. B7‑H4 negatively regulates T-cell mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. B7‑H4 is a single-pass type I membrane protein, which is over-expressed in breast, ovarian, endometrial, renal cell and non-small-cell lung cancers. The predicted molecular weight of B7‑H4 is 31 kDa. The glycosylated B7‑H4 is 50 to 80 kDa, and the non-glycosylated form is 28 kDa.