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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using B4GALT4 Polyclonal antibody (30478-1-AP)

    WB analysis using 30478-1-AP (same clone as 30478-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30478-1-AP (B4GALT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30478-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using B4GALT4 Polyclonal antibody (30478-1-AP)

    IF Staining of A549 using 30478-1-AP (same clone as 30478-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using B4GALT4 antibody (30478-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30478-1-PBS in a different storage buffer formulation.

B4GALT4 Polyclonal antibody, PBS Only

B4GALT4 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30478-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30478-1-PBS targets B4GALT4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag32337

Product name: Recombinant human B4GALT4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 39-142 aa of BC004523

Sequence: QEIPKAKEFMANFHKTLILGKGKTLTNEASTKKVELDNCPSVSPYLRGQSKLIFKPDLTLEEVQAENPKVSRGRYRPEECKALQRVAILVPHRNREKHLMYLLE

相同性解析による交差性が予測される生物種
Full Name UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4
Calculated molecular weight 40 kDa
Observed molecular weight34-40 kDa
GenBank accession numberBC004523
Gene Symbol B4GALT4
Gene ID (NCBI) 8702
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60513
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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