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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using B4GALT3 Polyclonal antibody (11041-1-AP)

    WB analysis using 11041-1-AP (same clone as 11041-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11041-1-AP (B4GALT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using B4GALT3 Polyclonal antibody (11041-1-AP)

    IHC staining of human liver cancer using 11041-1-AP (same clone as 11041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using B4GALT3 Polyclonal antibody (11041-1-AP)

    IHC staining of human liver cancer using 11041-1-AP (same clone as 11041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using B4GALT3 Polyclonal antibody (11041-1-AP)

    IHC staining of human breast cancer using 11041-1-AP (same clone as 11041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using B4GALT3 Polyclonal antibody (11041-1-AP)

    IHC staining of human breast cancer using 11041-1-AP (same clone as 11041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using B4GALT3 Polyclonal antibody (11041-1-AP)

    IF Staining of A431 using 11041-1-AP (same clone as 11041-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 11041-1-AP (B4GALT3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using B4GALT3 Polyclonal antibody (11041-1-AP)

    IF Staining of MCF-7 using 11041-1-AP (same clone as 11041-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using B4GALT3 antibody (11041-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

B4GALT3 Polyclonal antibody, PBS Only

B4GALT3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11041-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

b4Gal T3, b4Gal-T3, Beta 1,4 GalTase 3, Beta-1,4-galactosyltransferase 3, Beta-1,4-GalTase 3

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11041-1-PBS targets B4GALT3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1487

Product name: Recombinant human B4GALT3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-321 aa of BC009985

Sequence: MLRRLLERPCTLALLVGSQLAVMMYLSLGGFRSLSALFGRDQGPTFDYSHPRDVYSNLSHLPGAPGGPPAPQGLPYCPERSPLLVGPVSVSFSPVPSLAEIVERNPRVEPGGRYRPAGCEPRSRTAIIVPHRAREHHLRLLLYHLHPFLQRQQLAYGIYVIHQAGNGTFNRAKLLNVGVREALRDEEWDCLFLHDVDLLPENDHNLYVCDPRGPRHVAVAMNKFGYSLPYPQYFGGVSALTPDQYLKMNGFPNEYWGWGGEDDDIATRVRLAGMKISRPPTSVGHYKMVKHRGDKGNEENPHRFDLLVRTQNSWTQDGMNS

相同性解析による交差性が予測される生物種
Full Name UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3
Calculated molecular weight 44 kDa
Observed molecular weight44 kDa
GenBank accession numberBC009985
Gene Symbol B4GALT3
Gene ID (NCBI) 8703
RRIDAB_2290072
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60512
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

B4GALT3 is an enzyme responsible for the generation of poly-N-acetyllactosamine. The B4GALTs transfer galactose from UDP-Gal to N-acetylglucosamine (GlcNAc)-terminated oligosaccharides on N-glycan, O-glycan, or glycolipid to form N-acetyllactosamin (PMID: 23444218). B4GALT3 has been found to regulate cancer cell invasion (PMID: 25659296). B4GALT3 knockdown increased cell migration, invasion and the activation of β1 integrin and its downstream signaling (PMID: 24403309).

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