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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

B4GALNT2 Polyclonal antibody

B4GALNT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24024-1-AP

Synonyms

B4GALGT2, B4GALNT2, B4GALT, Cad, GALGT2, SD, Sda

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 24024-1-AP

    WB analysis of Jurkat using 24024-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24024-1-AP (B4GALNT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of COLO 320 using 24024-1-AP

    WB analysis of COLO 320 using 24024-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 24024-1-AP (B4GALNT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 24024-1-AP

    IHC staining of human colon cancer using 24024-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24024-1-AP (B4GALNT2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon cancer using 24024-1-AP

    IHC staining of human colon cancer using 24024-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24024-1-AP (B4GALNT2 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human colon using 24024-1-AP

    IHC staining of human colon using 24024-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24024-1-AP (B4GALNT2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon using 24024-1-AP

    IHC staining of human colon using 24024-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24024-1-AP (B4GALNT2 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inJurkat cells, COLO 320 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24024-1-AP is B4GALNT2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B4GALNT2 fusion protein Ag21166 相同性解析による交差性が予測される生物種
Full Name beta-1,4-N-acetyl-galactosaminyl transferase 2
Calculated molecular weight 566 aa, 63 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC113677
Gene symbol B4GALNT2
Gene ID (NCBI) 124872
RRIDAB_2879405
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

B4GALNT2, also named as GALGT2, catalyzes the last step in the biosynthesis of the human Sd(a) antigen, which is found on more than 90% of Caucasian red blood cells. It also catalyzes the last step in the biosynthesis of the Cad antigen, which is related both serologically and biochemically to the Sd(a) antigen. B4GALNT2 transfers a beta-1,4-linked GalNAc to the galactose residue of an alpha-2,3-sialylated chain. This antibody detects the 55-65 kDa B4GALNT2 protein.

Protocols

Product Specific Protocols
WB protocol for B4GALNT2 antibody 24024-1-APDownload protocol
IHC protocol for B4GALNT2 antibody 24024-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols