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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using B3GNT7 Polyclonal antibody (22879-1-AP)

    WB analysis of Jurkat using 22879-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22879-1-AP (B3GNT7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using B3GNT7 Polyclonal antibody (22879-1-AP)

    WB analysis of COLO 320 using 22879-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 22879-1-AP (B3GNT7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse placenta tissue using B3GNT7 Polyclonal antibody (22879-1-AP)

    WB analysis of mouse placenta using 22879-1-AP

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 22879-1-AP (B3GNT7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using B3GNT7 Polyclonal antibody (22879-1-AP)

    IHC staining of human colon cancer using 22879-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22879-1-AP (B3GNT7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using B3GNT7 Polyclonal antibody (22879-1-AP)

    IHC staining of human placenta using 22879-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22879-1-AP (B3GNT7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using B3GNT7 Polyclonal antibody (22879-1-AP)

    IHC staining of human stomach cancer using 22879-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22879-1-AP (B3GNT7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using B3GNT7 Polyclonal antibody (22879-1-AP)

    IHC staining of rat colon using 22879-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 22879-1-AP (B3GNT7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using B3GNT7 Polyclonal antibody (22879-1-AP)

    IHC staining of rat pancreas using 22879-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 22879-1-AP (B3GNT7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using B3GNT7 Polyclonal antibody (22879-1-AP)

    IHC staining of rat stomach using 22879-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 22879-1-AP (B3GNT7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using B3GNT7 Polyclonal antibody (22879-1-AP)

    IHC staining of mouse colon using 22879-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22879-1-AP (B3GNT7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using B3GNT7 Polyclonal antibody (22879-1-AP)

    IHC staining of human placenta using 22879-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22879-1-AP (B3GNT7 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using B3GNT7 Polyclonal antibody (22879-1-AP)

    IHC staining of human placenta using 22879-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22879-1-AP (B3GNT7 Antibody) at dilution of 1:50 (under 40x lens).

B3GNT7 Polyclonal antibody

B3GNT7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22879-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

Beta 1,3 Gn T7, Beta-1,3-Gn-T7, Beta-1,3-N-acetylglucosaminyltransferase 7, beta3GnT7, Beta3Gn-T7

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, COLO 320 cells, mouse placenta tissue
Positive IHC detected inhuman stomach cancer tissue, mouse colon tissue, rat stomach tissue, human colon cancer tissue, human placenta tissue, rat colon tissue, rat pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

22879-1-AP targets B3GNT7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18890

Product name: Recombinant human B3GNT7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 31-164 aa of BC148680

Sequence: PGQFLQEPPPPTLEPQKAQKPNGQLVNPNNFWKNPKDVAAPTPMASQGPQAWDVTTTNCSANINLTHQPWFQVLEPQFRQFLFYRHCRYFPMLLNHPEKCRGDVYLLVVVKSVITQHDRREAIRQTWGRERQSA

相同性解析による交差性が予測される生物種
Full Name UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7
Calculated molecular weight 330 aa, 37 kDa
Observed molecular weight 40-46 kDa
GenBank accession numberBC148680
Gene Symbol B3GNT7
Gene ID (NCBI) 93010
RRIDAB_2879182
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFL0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

B3GNT7, the glycosyltransferase gene that was most significantly upregulated by IL-22 treatment, encodes an N-acetylglucosaminyltransferase involved in the biosynthesis of polyLacNAc repeats of keratan sulfate (PMID: 34864058). B3GNT7 was initially identified in 2002 and is notable for high expression in healthy intestinal epithelial cells (PMID: 38886669). Overexpressed B3GNT7 were correlated with poor prognosis in breast cancer patients based on public datasets. B3GNT7 was shown to be a potential biomarker for unfavorable outcomes and therapeutic target of breast cancer (PMID: 37740763).

Protocols

Product Specific Protocols
IHC protocol for B3GNT7 antibody 22879-1-APDownload protocol
WB protocol for B3GNT7 antibody 22879-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanIF

Clin Exp Med

B3GNT7 preserves intestinal barrier integrity: a key defense mechanism against acute pancreatitis progression.

Authors - Jia Hu
View Article
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