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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using B3GNT7 Polyclonal antibody (22879-1-AP)

    WB analysis of Jurkat using 22879-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22879-1-AP (B3GNT7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using B3GNT7 Polyclonal antibody (22879-1-AP)

    WB analysis of COLO 320 using 22879-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 22879-1-AP (B3GNT7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse placenta tissue using B3GNT7 Polyclonal antibody (22879-1-AP)

    WB analysis of mouse placenta using 22879-1-AP

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 22879-1-AP (B3GNT7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using B3GNT7 Polyclonal antibody (22879-1-AP)

    IHC staining of human colon cancer using 22879-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22879-1-AP (B3GNT7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using B3GNT7 Polyclonal antibody (22879-1-AP)

    IHC staining of human placenta using 22879-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22879-1-AP (B3GNT7 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using B3GNT7 Polyclonal antibody (22879-1-AP)

    IHC staining of human placenta using 22879-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22879-1-AP (B3GNT7 Antibody) at dilution of 1:50 (under 40x lens).

B3GNT7 Polyclonal antibody

B3GNT7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22879-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

BGnT7, Beta3Gn-T7, beta3GnT7, Beta-1,3-N-acetylglucosaminyltransferase 7, Beta-1,3-Gn-T7

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, COLO 320 cells, mouse placenta tissue
Positive IHC detected inhuman colon cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22879-1-AP targets B3GNT7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18890

Product name: Recombinant human B3GNT7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 31-164 aa of BC148680

Sequence: PGQFLQEPPPPTLEPQKAQKPNGQLVNPNNFWKNPKDVAAPTPMASQGPQAWDVTTTNCSANINLTHQPWFQVLEPQFRQFLFYRHCRYFPMLLNHPEKCRGDVYLLVVVKSVITQHDRREAIRQTWGRERQSA

相同性解析による交差性が予測される生物種
Full Name UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7
Calculated molecular weight 330 aa, 37 kDa
Observed molecular weight 40-46 kDa
GenBank accession numberBC148680
Gene Symbol B3GNT7
Gene ID (NCBI) 93010
RRIDAB_2879182
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFL0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

B3GNT7, the glycosyltransferase gene that was most significantly upregulated by IL-22 treatment, encodes an N-acetylglucosaminyltransferase involved in the biosynthesis of polyLacNAc repeats of keratan sulfate (PMID: 34864058). B3GNT7 was initially identified in 2002 and is notable for high expression in healthy intestinal epithelial cells (PMID: 38886669). Overexpressed B3GNT7 were correlated with poor prognosis in breast cancer patients based on public datasets. B3GNT7 was shown to be a potential biomarker for unfavorable outcomes and therapeutic target of breast cancer (PMID: 37740763).

Protocols

Product Specific Protocols
IHC protocol for B3GNT7 antibody 22879-1-APDownload protocol
WB protocol for B3GNT7 antibody 22879-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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