Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse colon tissue, mouse small intestine tissue, mouse testis tissue |
| Positive IHC detected in | human colon cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
21291-1-AP targets B3GNT6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15825 Product name: Recombinant human B3GNT6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 102-343 aa of BC103910 Sequence: PLLWDAPAKCAGGRGVFLLLAVKSAPEHYERRELIRRTWGQERSYGGRPVRRLFLLGTPGPEDEARAERLAELVALEAREHGDVLQWAFADTFLNLTLKHLHLLDWLAARCPHARFLLSGDDDVFVHTANVVRFLQAQPPGRHLFSGQLMEGSVPIRDSWSKYFVPPQLFPGSAYPVYCSGGGFLLSGPTARALRAAARHTPLFPIDDAYMGMCLERAGLAPSGHEGIRPFGVQLPGAQQSS 相同性解析による交差性が予測される生物種 |
| Full Name | UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6 (core 3 synthase) |
| Calculated molecular weight | 384 aa, 43 kDa |
| Observed molecular weight | 43 kDa |
| GenBank accession number | BC103910 |
| Gene Symbol | B3GNT6 |
| Gene ID (NCBI) | 192134 |
| RRID | AB_2878834 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZMB0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The B3GNT6 protein, also known as core 3 synthase, is a member of the O-GlcNAc transferase (OGT) family and adds an N-acetylglucosamine to N-acetylgalactosamine-modifed serine or threonine. The B3GNT6 protein is responsible for the formation of the core 3 structure of O-glycans, which are important components of mucin-type glycoproteins. This process is known as the protein O-GlcNAcylation modifcation. B3GNT6 expression has been reported to be largely downregulated in gastric and colorectal cancers. B3GNT6 has 3 isoforms with the molecular mass of 29 and 43 kDa. (PMID: 35387659)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for B3GNT6 antibody 21291-1-AP | Download protocol |
| WB protocol for B3GNT6 antibody 21291-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogene Core 3 mucin-type O-glycan restoration in colorectal cancer cells promotes MUC1/p53/miR-200c-dependent epithelial identity. | ||
Cancer Med G-quadruplex-enhanced circular single-stranded DNA (G4-CSSD) adsorption of miRNA to inhibit colon cancer progression | ||
Int J Food Sci Nutr Ellagic acid alleviates TNBS-induced intestinal barrier dysfunction by regulating mucin secretion and maintaining tight junction integrity in rats | ||
Cancer Med IGF2BP2 promotes pancreatic carcinoma progression by enhancing the stability of B3GNT6 mRNA via m6A methylation
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