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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

B3GNT5 Polyclonal antibody

B3GNT5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20422-1-AP

Synonyms

B3GN T5, B3GNT5, Beta 1,3 Gn T5, beta3Gn T5, BGnT 5, Lactotriaosylceramide synthase, Lc(3)Cer synthase, Lc3 synthase

Validation Data Gallery

Applications:
  • WB analysis of mouse lung using 20422-1-AP

    WB analysis of mouse lung using 20422-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 20422-1-AP (B3GNT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse lung using 20422-1-AP

    WB analysis of mouse lung using 20422-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 20422-1-AP (B3GNT5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 20422-1-AP

    IHC staining of human lung using 20422-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20422-1-AP (B3GNT5 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung using 20422-1-AP

    IHC staining of human lung using 20422-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20422-1-AP (B3GNT5 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20422-1-AP is B3GNT5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B3GNT5 fusion protein Ag14246 相同性解析による交差性が予測される生物種
Full Name UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5
Calculated molecular weight 378 aa, 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC028058
Gene symbol B3GNT5
Gene ID (NCBI) 84002
RRIDAB_10694280
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for B3GNT5 antibody 20422-1-APDownload protocol
IHC protocol for B3GNT5 antibody 20422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Cell Mol Med

MiR-30a-5p inhibits proliferation and metastasis of hydatidiform mole by regulating B3GNT5 through ERK/AKT pathways.

Authors - Zhenzhen Guo
View Article
humanWB

Cell Rep

Sequential genome-wide CRISPR-Cas9 screens identify genes regulating cell-surface expression of tetraspanins

Authors - Jicheng Yang
View Article