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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using B3GAT1 Monoclonal antibody (66711-1-Ig)

    WB analysis using 66711-1-Ig (same clone as 66711-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66711-1-Ig (B3GAT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66711-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using B3GAT1 Monoclonal antibody (66711-1-Ig)

    WB analysis of mouse brain using 66711-1-Ig (same clone as 66711-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66711-1-Ig (B3GAT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66711-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K-562 cells using B3GAT1 Monoclonal antibody (66711-1-Ig)

    FC experiment of K-562 using 66711-1-Ig (same clone as 66711-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human CD57 (66711-1-Ig, Clone:1F4A11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66711-1-PBS in a different storage buffer formulation.

B3GAT1 Monoclonal antibody, PBS Only

B3GAT1 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 66711-1-PBS
Clone No.1F4A11

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

1F4A11, Beta-1,3-glucuronyltransferase 1, EC:2.4.1.135, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, GLCATP

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66711-1-PBS targets B3GAT1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag13593

Product name: Recombinant human CD57 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 25-334 aa of BC010466

Sequence: HQSTLAPLLAVHKDEGSDPRRETPPGADPREYCTSDRDIVEVVRTEYVYTRPPPWSDTLPTIHVVTPTYSRPVQKAELTRMANTLLHVPNLHWLVVEDAPRRTPLTARLLRDTGLNYTHLHVETPRNYKLRGDARDPRIPRGTMQRNLALRWLRETFPRNSSQPGVVYFADDDNTYSLELFEEMRSTRRVSVWPVAFVGGLRYEAPRVNGAGKVVGWKTVFDPHRPFAIDMAGFAVNLRLILQRSQAYFKLRGVKGGYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPSVEI

相同性解析による交差性が予測される生物種
Full Name beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa, 100 kDa
GenBank accession numberBC010466
Gene Symbol B3GAT1
Gene ID (NCBI) 27087
RRIDAB_2882062
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9P2W7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment. The calculated molecular weight of B3GAT1 is 38 kDa and 66711-1-Ig antibody detectes 100 kDa form in SDS-PAGE which might as a result of homodimer or glycosylation.

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