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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

B3GALTL Polyclonal antibody

B3GALTL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14601-1-AP

Synonyms

B3GALTL, B3Glc T, B3GLCT, B3GTL, Beta 1,3 glucosyltransferase, beta3Glc T, Gal T

Validation Data Gallery

Applications:
  • WB analysis of human heart using 14601-1-AP

    WB analysis of human heart using 14601-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14601-1-AP (B3GALTL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse skeletal muscle using 14601-1-AP

    WB analysis of mouse skeletal muscle using 14601-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14601-1-AP (B3GALTL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human endometrial cancer using 14601-1-AP

    IHC staining of human endometrial cancer using 14601-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14601-1-AP (B3GALTL antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human endometrial cancer using 14601-1-AP

    IHC staining of human endometrial cancer using 14601-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14601-1-AP (B3GALTL antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman heart tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14601-1-AP targets B3GALTL in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B3GALTL fusion protein Ag6146 相同性解析による交差性が予測される生物種
Full Name beta 1,3-galactosyltransferase-like
Calculated molecular weight 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC068595
Gene symbol B3GALTL
Gene ID (NCBI) 145173
RRIDAB_2063186
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for B3GALTL antibody 14601-1-APDownload protocol
IHC protocol for B3GALTL antibody 14601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols