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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

B3GALNT2 Polyclonal antibody

B3GALNT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17142-1-AP

Synonyms

B3GalNAc T2, B3GALNT2, Beta 1,3 GalNAc T2

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 17142-1-AP

    WB analysis of HEK-293 using 17142-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17142-1-AP (B3GALNT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 17142-1-AP

    IHC staining of human heart using 17142-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17142-1-AP (B3GALNT2 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human heart using 17142-1-AP

    IHC staining of human heart using 17142-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17142-1-AP (B3GALNT2 antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17142-1-AP targets B3GALNT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B3GALNT2 fusion protein Ag10907 相同性解析による交差性が予測される生物種
Full Name beta-1,3-N-acetylgalactosaminyltransferase 2
Calculated molecular weight 500 aa, 57 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC029564
Gene symbol B3GALNT2
Gene ID (NCBI) 148789
RRIDAB_2063040
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for B3GALNT2 antibody 17142-1-APDownload protocol
IHC protocol for B3GALNT2 antibody 17142-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

J Hematol Oncol

Increased B3GALNT2 in hepatocellular carcinoma promotes macrophage recruitment via reducing acetoacetate secretion and elevating MIF activity.

Authors - Tianxiao Yang
View Article