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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using B3GALNT1 Polyclonal antibody (22048-1-AP)

    WB analysis using 22048-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22048-1-AP (B3GALNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse placenta tissue using B3GALNT1 Polyclonal antibody (22048-1-AP)

    WB analysis of mouse placenta using 22048-1-AP

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 22048-1-AP (B3GALNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using B3GALNT1 Polyclonal antibody (22048-1-AP)

    IHC staining of human heart using 22048-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 22048-1-AP (B3GALNT1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using B3GALNT1 Polyclonal antibody (22048-1-AP)

    IHC staining of human heart using 22048-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 22048-1-AP (B3GALNT1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using B3GALNT1 Polyclonal antibody (22048-1-AP)

    IHC staining of human kidney using 22048-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 22048-1-AP (B3GALNT1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using B3GALNT1 Polyclonal antibody (22048-1-AP)

    IHC staining of human kidney using 22048-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 22048-1-AP (B3GALNT1 antibody) at dilution of 1:50 (under 40x lens).

B3GALNT1 Polyclonal antibody

B3GALNT1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22048-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

b3Gal T3, B3GALNT1, B3GALT3, Beta 1,3 GalNAc T1, Beta 1,3 GalTase 3, Beta 3 Gx T3, beta3Gal T3, Beta3GalT3, galT3, Gb4Cer, GLCT3, GLOB, Globoside synthase, P1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse placenta tissue, MCF-7 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22048-1-AP targets B3GALNT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16976

Product name: Recombinant human B3GALNT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 43-331 aa of BC047618

Sequence: VIERVNWMYFYEYEPIYRQDFHFTLREHSNCSHQNPFLVILVTSHPSDVKARQAIRVTWGEKKSWWGYEVLTFFLLGQEAEKEDKMLALSLEDEHLLYGDIIRQDFLDTYNNLTLKTIMAFRWVTEFCPNAKYVMKTDTDVFINTGNLVKYLLNLNHSEKFFTGYPLIDNYSYRGFYQKTHISYQEYPFKVFPPYCSGLGYIMSRDLVPRIYEMMGHVKPIKFEDVYVGICLNLLKVNIHIPEDTNLFFLYRIHLDVCQLRRVIAAHGFSSKEIITFWQVMLRNTTCHY

相同性解析による交差性が予測される生物種
Full Name beta-1,3-N-acetylgalactosaminyltransferase 1 (globoside blood group)
Calculated molecular weight 40 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC047618
Gene Symbol B3GALNT1
Gene ID (NCBI) 8706
RRIDAB_11182184
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75752
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for B3GALNT1 antibody 22048-1-APDownload protocol
IHC protocol for B3GALNT1 antibody 22048-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols