AuthentiKine™ Human FLT3 Ligand ELISA Kit0 Publications

カタログ番号: KE00197

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
AuthentiKine™ Human FLT3 Ligand ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, plasma, Cell culture supernatants

アッセイ方法:
Sandwich

感度:
0.15 pg/mL

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human plasma 89 81-105
Cell culture supernatants 89 76-101

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 511.8 13.4 2.6
2 20 124.7 2.9 2.3
3 20 28.2 1.2 4.3

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 517.1 17.3 3.3
2 24 123.4 4.6 3.7
3 24 28.5 1.5 5.1

製品概要
KE00197 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The FLT3 Ligand ELISA kit is to be used to detect and quantify protein levels of endogenous FLT3 Ligand. The assay recognizes human FLT3 Ligand. An antibody specific for FLT3 Ligand has been pre-coated onto the microwells. TheFLT3 Ligand protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for FLT3 Ligand is added to detect the captured FLT3 Ligand protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
FL, Flt3 ligand, Flt3L, FLT3LG, SL cytokine
背景説明

FLT3 Ligand is a member of a small family of growth factors that stimulate the proliferation of hematopoietic cell. FLT3 ligand is known to induce profound expansion of dendritic cells (DCs) in the peripheral lymphoid organs, and FLT3 ligand-induced DCs have an immature phenotype. Its receptor, FLT3, is expressed on myeloid, lymphoid and dendritic cell progenitors and is considered an important growth and differentiation factor for several hematopoietic lineages. Activating mutations of FLT3 are frequently found in acute myeloid leukemia (AML) patients and associated with a poor clinical prognosis. Serum FLT3 Ligand is a biomarker of progenitor cell mass and prognosis in acute myeloid leukemia.


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