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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ataxin-3/ATXN3 Recombinant monoclonal antibody (87817-1-RR)

    WB analysis using 87817-1-RR (same clone as 87817-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87817-1-RR (Ataxin-3/ATXN3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87817-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Ataxin-3/ATXN3 Recombinant monoclonal antibody (87817-1-RR)

    WB analysis using 87817-1-RR (same clone as 87817-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87817-1-RR (MU154079-4(ATXN3) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87817-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using Ataxin-3/ATXN3 Recombinant monoclonal antibody (87817-1-RR)

    WB analysis of MCF-7 using 87817-1-RR (same clone as 87817-1-PBS)

    WB result of Ataxin-3/ATXN3 antibody (87817-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ataxin-3/ATXN3 transfected MCF-7 cells. This data was developed using the same antibody clone with 87817-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bowen disease tissue using Ataxin-3/ATXN3 Recombinant monoclonal antibody (87817-1-RR)

    IHC staining of human bowen disease using 87817-1-RR (same clone as 87817-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease tissue slide using 87817-1-RR (Ataxin-3/ATXN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87817-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bowen disease tissue using Ataxin-3/ATXN3 Recombinant monoclonal antibody (87817-1-RR)

    IHC staining of human bowen disease using 87817-1-RR (same clone as 87817-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease tissue slide using 87817-1-RR (Ataxin-3/ATXN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87817-1-PBS in a different storage buffer formulation.

  • Affinity of 87817-1-RR

    Affinity of 87817-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87817-1-RR against Human Ataxin-3/ATXN3 were performed. The affinity constant is below 1 pM.

Ataxin-3/ATXN3 Recombinant monoclonal antibody, PBS Only

Ataxin-3/ATXN3 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 87817-1-PBS
Clone No.253467D4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ATX3,ATXN3, ATXN3, AT3, ataxin 3, Ataxin-3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87817-1-PBS targets Ataxin-3/ATXN3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag41832

Product name: Recombinant human MU154079-4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-361 aa of

Sequence: MSKIKMESIFHEKQEGSLCAQHCLNNLLQGEYFSPVELSSIAHQLDEEERMRMAEGGVTSEDYRTFLQQPSGNMDDSGFFSIQVISNALKVWGLELILFNSPEYQRLRIDPINERSFICNYKEHWFTVRKLGKQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVVKGDLPDCEADQLLQMIRVQQMHRPKLIGEELAQLKEQRVHKTDLERVLEANDGSGMLDEDEEDLQRALALSRQEIDMEDEEADLRRAIQLSMQGSSRNISQDMTQTSGTNLTSEELRKRREAYFEKQQQKQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQGDLSGQSSHPCERPATSSGALGSDLGDAMSEEDMLQAAVTMSLETVRNDLKTEGKKHHHHHHHH

相同性解析による交差性が予測される生物種
Full Name ataxin 3
Observed molecular weight 35-42 kDa
Gene Symbol ATXN3
Gene ID (NCBI) 4287
RRIDAB_3745767
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP54252
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.

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