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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ataxin 2 Recombinant antibody (80919-2-RR)

    WB analysis using 80919-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80919-2-RR (Ataxin 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Ataxin 2 Recombinant antibody (80919-2-RR)

    WB analysis using 80919-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80919-2-RR (Ataxin 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using Ataxin 2 Recombinant antibody (80919-2-RR)

    IP experiment of NIH/3T3 using 80919-2-RR

    IP result of anti-Ataxin 2 (IP:80919-2-RR, 4ug; Detection:80919-2-RR 1:1000) with NIH/3T3 cells lysate 1200 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Ataxin 2 Recombinant antibody (80919-2-RR)

    IHC staining of mouse brain using 80919-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80919-2-RR (Ataxin 2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Ataxin 2 Recombinant antibody (80919-2-RR)

    IF Staining of mouse brain using 80919-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Ataxin 2 antibody (80919-2-RR, Clone: 250888E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 80919-2-RR

    Affinity and Kinetic Characterization of 80919-2-RR

    Biolayer interferometry (BLl) kinetic assays of 80919-2-RR against Human Ataxin 2 were performed. The affinity constant is 0.153 nM.

Ataxin 2 Recombinant monoclonal antibody

Ataxin 2 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 80919-2-RR
Clone No.250888E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

ATXN2, ataxin2, Ataxin-2, ATX2, ATXN 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, NIH/3T3 cells, Neuro-2a cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80919-2-RR targets Ataxin 2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag16470

Product name: Recombinant human Ataxin 2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 251-600 aa of BC114546

Sequence: GSDQRVVNGGVPWPSPCPSPSSRPPSRYQSGPNSLPPRAATPTRPPSRPPSRPSRPPSHPSAHGSPAPVSTMPKRMSSEGPPRMSPKAQRHPRNHRVSAGRGSISSGLEFVSHNPPSEAATPPVARTSPSGGTWSSVVSGVPRLSPKTHRPRSPRQNSIGNTPSGPVLASPQAGIIPTEAVAMPIPAASPTPASPASNRAVTPSSEAKDSRLQDQRQNSPAGNKENIKPNETSPSFSKAENKGISPVVSEHRKQIDDLKKFKNDFRLQPSSTSESMDQLLNKNREGEKSRDLIKDKIEPSAKDSFIENSSSNCTSGSSKPNSPSISPSILSNTEHKRGPEVTSQGVQTSS

相同性解析による交差性が予測される生物種
Full Name ataxin 2
Calculated molecular weight 1313 aa, 140 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberBC114546
Gene Symbol ATXN2
Gene ID (NCBI) 6311
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ99700
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATXN2 contains a repeat structure with 22 or 23 triplets coding for glutamine and the (CAG)8CAA(CAG)4CAA(CAG)8 sequence; expansion of this domain to a size ≥34 triplets with a pure CAG sequence primarily causes autosomal dominant SCA2 [PMID:18418684], while ATXN2 expansions with CAA interruptions were observed as the cause of Levo-dopa responsive Parkinson's disease [PMID:10668726]. ATXN2 expansions associated with ALS were reported to be interrupted by at least one CAA triplet [PMID:21537950]

Protocols

Product Specific Protocols
WB protocol for Ataxin 2 antibody 80919-2-RRDownload protocol
IHC protocol for Ataxin 2 antibody 80919-2-RRDownload protocol
IF protocol for Ataxin 2 antibody 80919-2-RRDownload protocol
IP protocol for Ataxin 2 antibody 80919-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols