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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Arginase-2 Recombinant antibody (84808-5-RR)

    WB analysis using 84808-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84808-5-RR (Arginase-2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. Mouse liver tissue as a negative control.

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Arginase-2 Recombinant antibody

Arginase-2 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84808-5-RR
Clone No.242362A9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

ARG2, Arginase-2, mitochondrial, arginase, type II, Arginase II, ARG 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU-251 cells, K-562 cells, Caco-2 cells,

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84808-5-RR targets Arginase-2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Arginase-2 fusion protein Ag6609 相同性解析による交差性が予測される生物種
Full Name arginase, type II
Calculated molecular weight 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC001350
Gene Symbol ARG2
Gene ID (NCBI) 384
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP78540
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Arginase 2 is composed of 354 amino acid residues, including an NH2-terminal presequence for mitochondrial targeting and import. In the mitochondria, ornithine generated by Arginase 2 will give rise to glutamate via ornithine aminotransferase (OAT). Glutamate participates in several transamination reactions, including forming α-ketoglutarate (αKG) that may enter the TCA cycle and increase cycle intermediates and flux.Arginase 1 is mainly expressed in hepatocytes, and mice with a disruption of Arginase 1 gene die soon after birth. Arginase 2 is poorly expressed in hepatocytes, and most highly expressed in kidney, prostate, and immune cells such as monocyte/ macrophages. (PMID: 25234945,PMID: 27214549)

Protocols

Product Specific Protocols
WB protocol for Arginase-2 antibody 84808-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols