Validation Data Gallery

Filter: AllWBIPIHCIF-P

WB analysis using 16473-1-AP (same clone as 16473-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16473-1-PBS in a different storage buffer formulation.
WB analysis using 16473-1-AP (same clone as 16473-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16473-1-PBS in a different storage buffer formulation.
WB analysis of mouse heart using 16473-1-AP (same clone as 16473-1-PBS)
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16473-1-PBS in a different storage buffer formulation.
WB analysis of SKOV-3 using 16473-1-AP (same clone as 16473-1-PBS)
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16473-1-PBS in a different storage buffer formulation.
WB analysis of mouse kidney using 16473-1-AP (same clone as 16473-1-PBS)
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16473-1-PBS in a different storage buffer formulation.
IP experiment of mouse heart using 16473-1-AP (same clone as 16473-1-PBS)
IP result of anti-Aquaporin 4 (IP:16473-1-AP, 4ug; Detection:16473-1-AP 1:300) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 16473-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 16473-1-AP (same clone as 16473-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16473-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 16473-1-AP (same clone as 16473-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16473-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 16473-1-AP (same clone as 16473-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 16473-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 16473-1-AP (same clone as 16473-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 16473-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 16473-1-AP (same clone as 16473-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16473-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 16473-1-AP (same clone as 16473-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16473-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16473-1-AP (same clone as 16473-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16473-1-AP (Aquaporin 4 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 16473-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16473-1-AP (same clone as 16473-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16473-1-AP (Aquaporin 4 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 16473-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 16473-1-AP (same clone as 16473-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16473-1-AP (Aquaporin 4 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16473-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 16473-1-AP (same clone as 16473-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16473-1-AP (Aquaporin 4 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16473-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 16473-1-AP (same clone as 16473-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Aquaporin 4 antibody (16473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), GFAP antibody (60190-1-Ig, Clone: 4B2E10, red). This data was developed using the same antibody clone with 16473-1-PBS in a different storage buffer formulation.
IF Staining of rat brain using 16473-1-AP (same clone as 16473-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Aquaporin 4 antibody (16473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), GFAP antibody (60190-1-Ig, Clone: 4B2E10, red). This data was developed using the same antibody clone with 16473-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Aquaporin 4 Polyclonal antibody, PBS Only

Aquaporin 4 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 16473-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

AQP4, AQP 4, AQP-4, Aquaporin4, Aquaporin-4

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16473-1-PBS targets Aquaporin 4 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9561 Product name: Recombinant human AQP4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 208-323 aa of BC022286 Sequence: TGASMNPARSFGPAVIMGNWENHWIYWVGPIIGAVLAGGLYEYVFCPDVEFKRRFKEAFSKAAQQTKGSYMEVEDNRSQVETDDLILKPGVVHVIDVDRGEEKKGKDQSGEVLSSV 相同性解析による交差性が予測される生物種
Full Name	Aquaporin 4
Calculated molecular weight	323 aa, 35 kDa
Observed molecular weight	35-37 kDa, 32-34 kDa
GenBank accession number	BC022286
Gene Symbol	Aquaporin 4
Gene ID (NCBI)	361
RRID	AB_2827426
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P55087
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16473-1-AP (same clone as 16473-1-PBS)

WB analysis using 16473-1-AP (same clone as 16473-1-PBS)

WB analysis of mouse heart using 16473-1-AP (same clone as 16473-1-PBS)

WB analysis of SKOV-3 using 16473-1-AP (same clone as 16473-1-PBS)

WB analysis of mouse kidney using 16473-1-AP (same clone as 16473-1-PBS)

IP experiment of mouse heart using 16473-1-AP (same clone as 16473-1-PBS)

IHC staining of mouse brain using 16473-1-AP (same clone as 16473-1-PBS)

IHC staining of mouse brain using 16473-1-AP (same clone as 16473-1-PBS)

IHC staining of rat brain using 16473-1-AP (same clone as 16473-1-PBS)

IHC staining of rat brain using 16473-1-AP (same clone as 16473-1-PBS)

IHC staining of mouse heart using 16473-1-AP (same clone as 16473-1-PBS)