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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Apoe Polyclonal antibody (31454-1-AP)

    WB analysis using 31454-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31454-1-AP (Apoe antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Apoe Polyclonal antibody (31454-1-AP)

    WB analysis using 31454-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31454-1-AP (Apoe antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using Apoe Polyclonal antibody (31454-1-AP)

    IHC staining of rat cerebellum using 31454-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 31454-1-AP (Apoe antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using Apoe Polyclonal antibody (31454-1-AP)

    IHC staining of rat cerebellum using 31454-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 31454-1-AP (Apoe antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Apoe Polyclonal antibody (31454-1-AP)

    IF Staining of HepG2 using 31454-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Apoe antibody (31454-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Apoe Polyclonal antibody

Apoe Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31454-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

AI255918, apolipoprotein E

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU-251 cells, BV-2 cells, C2C12 cells, mouse brain tissue, mouse skeletal muscle tissue, mouse cerebellum tissue, rat brain tissue, pig cerebellum tissue
Positive IHC detected inrat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31454-1-AP targets Apoe in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Apoe fusion protein Eg1093 相同性解析による交差性が予測される生物種
Full Name Apolipoprotein E
Calculated molecular weight36kDa
Observed molecular weight31~36 kDa
GenBank accession numberNM_001305819.1
Gene Symbol Apoe
Gene ID (NCBI) 11816
RRIDAB_3669993
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP08226
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Apolipoprotein E (Apoe) is a core component of plasma lipoproteins that participates in diverse biological processes, including plasma lipoprotein metabolism, intracellular cholesterol utilization, cell growth, immunoregulation, and neuronal growth and repair (PMID: 17854398). Mouse apoE, like apoE4, contains the equivalent of Arg-112 and Glu-255, but lacks the critical Arg-61 equivalent (it contains Thr-61). Thus, mouse apoE does not display apoE4 domain interaction (PMID: 11553788).

Protocols

Product Specific Protocols
WB protocol for Apoe antibody 31454-1-APDownload protocol
IHC protocol for Apoe antibody 31454-1-APDownload protocol
IF protocol for Apoe antibody 31454-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols