Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30383-1-AP targets Antizyme inhibitor 1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag33058 Product name: Recombinant human AZIN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 124-213 aa of BC019279 Sequence: AKVGVNILTCDNEIELKKIARNHPNAKVLLHIATEDNIGGEEGNMKFGTTLKNCRHLLECAKELDVQIIGVKFHVSSACKESQVYVHALS 相同性解析による交差性が予測される生物種 |
| Full Name | antizyme inhibitor 1 |
| Calculated molecular weight | 49.5 kDa |
| Observed molecular weight | 40-50 kDa |
| GenBank accession number | BC019279 |
| Gene Symbol | AZIN1 |
| Gene ID (NCBI) | 51582 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14977 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AZIN1(Antizyme inhibitor 1) is also named as OAZI, OAZIN and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine in the first and apparently rate-limiting step in polyamine biosynthesis, and playing a role in the regulation of polyamine synthesis (negative regulator of cellular polyamines).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Antizyme inhibitor 1 antibody 30383-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
