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Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using Annexin A4 Monoclonal antibody (60288-2-Ig)

    WB analysis of COLO 320 using 60288-2-Ig (same clone as 60288-2-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60288-2-Ig (Annexin IV antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using Annexin A4 Monoclonal antibody (60288-2-Ig)

    WB analysis of human heart using 60288-2-Ig (same clone as 60288-2-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 60288-2-Ig (Annexin IV Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using Annexin A4 Monoclonal antibody (60288-2-Ig)

    WB analysis of human placenta using 60288-2-Ig (same clone as 60288-2-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 60288-2-Ig (Annexin IV antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using Annexin A4 Monoclonal antibody (60288-2-Ig)

    WB analysis of A549 using 60288-2-Ig (same clone as 60288-2-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 60288-2-Ig (Annexin IV antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Annexin A4 Monoclonal antibody (60288-2-Ig)

    IHC staining of human placenta using 60288-2-Ig (same clone as 60288-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60288-2-Ig (Annexin IV antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Annexin A4 Monoclonal antibody (60288-2-Ig)

    IHC staining of human placenta using 60288-2-Ig (same clone as 60288-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60288-2-Ig (Annexin IV antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Annexin A4 Monoclonal antibody (60288-2-Ig)

    IF Staining of HeLa using 60288-2-Ig (same clone as 60288-2-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60288-2-Ig(Annexin IV antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

Annexin A4 Monoclonal antibody, PBS Only

Annexin A4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60288-2-PBS
Clone No.1A10B5

Host / Isotype

Mouse / IgG2c

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

35-beta calcimedin, Annexin A4, Annexin IV, Annexin-4, ANXA4, Chromobindin-4, Endonexin I, Lipocortin IV

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60288-2-PBS targets Annexin A4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag0130

Product name: Recombinant human Annexin IV protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 33-202 aa of BC000182

Sequence: GTDEDAIISVLAYRNTAQRQEIRTAYKSTIGRDLIDDLKSELSGNFEQVIVGMMTPTVLYDVQELRRAMKGAGTDEGCLIEILASRTPEEIRRISQTYQQQYGRSLEDDIRSDTSFMFQRVLVSLSAGGRDEGNYLDDALVRQDAQDLYEAGEKKWGTDEVKFLTVLCSR

相同性解析による交差性が予測される生物種
Full Name annexin A4
Calculated molecular weight 36 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC000182
Gene Symbol Annexin A4
Gene ID (NCBI) 307
RRIDAB_2881405
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP09525
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Annexin A4 (Anxa4) is one of the Ca2 +-regulated and phospholipid-binding annexin superfamily proteins, and is located at 2p13. Anxa4 has a potential role in diagnosis, prognosis, and treatment of certain cancers. Annexin A4 shares 45 to 59% identity with other members and possibly interacts with ATP. Annexin A4 has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. The Annexin A4 protein is expressed in a wide range of tissues, associated with polarized epithelial cells and is proposed to be involved in the regulation of chloride ions across the epithelial membrane.

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