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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Annexin A3 Monoclonal antibody (66405-1-Ig)

    WB analysis using 66405-1-Ig (same clone as 66405-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Caco-2 cells using Annexin A3 Monoclonal antibody (66405-1-Ig)

    WB analysis of Caco-2 using 66405-1-Ig (same clone as 66405-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Annexin A3 Monoclonal antibody (66405-1-Ig)

    WB analysis using 66405-1-Ig (same clone as 66405-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using Annexin A3 Monoclonal antibody (66405-1-Ig)

    WB analysis of L02 using 66405-1-Ig (same clone as 66405-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Annexin A3 Monoclonal antibody (66405-1-Ig)

    WB analysis of HEK-293 using 66405-1-Ig (same clone as 66405-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Annexin A3 Monoclonal antibody (66405-1-Ig)

    IHC staining of human pancreas using 66405-1-Ig (same clone as 66405-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66405-1-Ig (Annexin A3 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Annexin A3 Monoclonal antibody (66405-1-Ig)

    IHC staining of human pancreas using 66405-1-Ig (same clone as 66405-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66405-1-Ig (Annexin A3 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

Annexin A3 Monoclonal antibody, PBS Only

Annexin A3 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66405-1-PBS
Clone No.1A11E4

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

ANXA3, 1A11E4, 35 alpha calcimedin, 35-alpha calcimedin, Annexin 3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66405-1-PBS targets Annexin A3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag2381

Product name: Recombinant human ANXA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-322 aa of BC000871

Sequence: MASIWVGHRGTVRDYPDFSPSVDAEAIQKAIRGIGTDEKMLISILTERSNAQRQLIVKEYQAAYGKELKDDLKGDLSGHFEHLMVALVTPPAVFDAKQLKKSMKGAGTNEDALIEILTTRTSRQMKDISQAYYTVYKKSLGDDISSETSGDFRKALLTLADGRRDESLKVDEHLAKQDAQILYKAGENRWGTDEDKFTEILCLRSFPQLKLTFDEYRNISQKDIVDSIKGELSGHFEDLLLAIVNCVRNTPAFLAERLHRALKGIGTDEFTLNRIMVSRSEIDLLDIRTEFKKHYGYSLYSAIKSDTSGDYEITLLKICGGD

相同性解析による交差性が予測される生物種
Full Name annexin A3
Calculated molecular weight 322 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000871
Gene Symbol Annexin A3
Gene ID (NCBI) 306
RRIDAB_2881779
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP12429
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Annexin A3 (Anxa3), also named as lipocortin3, placental anticoagulant protein3 (PAP-III), belongs to annexin family. Annexins are calcium-dependent phospholipids-binding high-abundant intracellular proteins. ANXA3 has been reported to play crucial roles in a diverse range of molecular and cellular processes, including ion channel regulation, anticoagulation, anti-inflammation, vesicle traffick-ing, signal transduction, cell migration, and cell proliferation. It also exhibits important roles in cancer progression, metastasis, and drug resistance.