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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Annexin A2 Monoclonal antibody (66035-1-Ig)

    WB analysis using 66035-1-Ig (same clone as 66035-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66035-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Annexin A2 Monoclonal antibody (66035-1-Ig)

    WB analysis using 66035-1-Ig (same clone as 66035-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66035-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Annexin A2 Monoclonal antibody (66035-1-Ig)

    WB analysis of HEK-293 using 66035-1-Ig (same clone as 66035-1-PBS)

    WB result of Annexin A2 antibody (66035-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin A2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using Annexin A2 Monoclonal antibody (66035-1-Ig)

    WB analysis of MCF-7 using 66035-1-Ig (same clone as 66035-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 66035-1-Ig (ANXA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using Annexin A2 Monoclonal antibody (66035-1-Ig)

    WB analysis of human brain using 66035-1-Ig (same clone as 66035-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66035-1-Ig (ANXA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Annexin A2 Monoclonal antibody (66035-1-Ig)

    WB analysis using 66035-1-Ig (same clone as 66035-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66035-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Annexin A2 Monoclonal antibody (66035-1-Ig)

    IHC staining of human breast cancer using 66035-1-Ig (same clone as 66035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Annexin A2 Monoclonal antibody (66035-1-Ig)

    IHC staining of human breast cancer using 66035-1-Ig (same clone as 66035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Annexin A2 Monoclonal antibody (66035-1-Ig)

    IHC staining of human skin cancer using 66035-1-Ig (same clone as 66035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Annexin A2 Monoclonal antibody (66035-1-Ig)

    IHC staining of human skin cancer using 66035-1-Ig (same clone as 66035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of T-47D cells using Annexin A2 Monoclonal antibody (66035-1-Ig)

    IF Staining of T-47D using 66035-1-Ig (same clone as 66035-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using 66035-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Annexin A2 Monoclonal antibody (66035-1-Ig)

    IF Staining of HeLa using 66035-1-Ig (same clone as 66035-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66035-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Annexin A2 Monoclonal antibody (66035-1-Ig)

    FC experiment of HeLa using 66035-1-Ig (same clone as 66035-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Annexin A2 Monoclonal antibody (66035-1-Ig, Clone:1C1E12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66035-1-PBS targets Annexin A2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Annexin A2 fusion protein Ag16654 相同性解析による交差性が予測される生物種
Full Name annexin A2
Calculated molecular weight 339 aa, 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC016774
Gene Symbol Annexin A2
Gene ID (NCBI) 302
ENSEMBL Gene IDENSG00000182718
RRIDAB_11045659
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07355
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.