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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Annexin A13 Recombinant antibody (85955-1-RR)

    WB analysis using 85955-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85955-1-RR (Annexin A13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85955-1-RR

    Affinity and Kinetic Characterization of 85955-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85955-1-RR against Human Annexin A13 were performed. The affinity constant is 6.29 nM.

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Annexin A13 Recombinant antibody

Annexin A13 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85955-1-RR
Clone No.250350A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ANXA13, Annexin 13, Annexin XIII, Annexin-13, ANX13

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse small intestine tissue, rat small intestine tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85955-1-RR targets Annexin A13 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Annexin A13 fusion protein Ag18581 相同性解析による交差性が予測される生物種
Full Name annexin A13
Calculated molecular weight 316 aa, 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC125158
Gene Symbol Annexin A13
Gene ID (NCBI) 312
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP27216
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Annexin A13 (ANXA13) is believed to be the original founder gene of the 12-member vertebrate annexin A family, and it has acquired an intestine-specific expression associated with a highly differentiated intracellular transport function (PMID: 11961095). ANXA13, which is highly expressed in the digestive system and believed to be the original founder gene of the annexin A family (PMID: 28423508). Limited evidence suggests that ANXA13 may play a role in apoptotic cell-mediated immunosuppression, hypertension, and cell death recognition (PMID: 28423508). ANXA13 is highly expressed in the human colon adenocarcinoma cell line HT29.

Protocols

Product Specific Protocols
WB protocol for Annexin A13 antibody 85955-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols